Ikhithi Yokuhlola I-Bioendo KC Endotoxin (I-Kinetic Chromogenic Assay)

Ikhithi Yokuhlola I-Bioendo KC Endotoxin (I-Kinetic Chromogenic Assay)

1. Ulwazi Lomkhiqizo

Kukhithi Yokuhlola I-Bioendo KC Endotoxin, i-Amebocyte Lysate ihlanganiswe ndawonye ne-chromogenic substrate.Ngakho-ke, i-endotoxin yebhaktheriya ingalinganiswa ngokusekelwe ekuphenduleni kwe-chromogenic.Isivivinyo siwukumelana okuqinile nokugxambukela, futhi sinezinzuzo ze-kinetic turbidimetric kanye ne-end-point chromogenic method.Ikhithi Yokuhlola ye-Bioendo Endotoxin iqukethe i-Chromogenic Amebocyte Lysate, i-Reconstitution Buffer, i-CSE, Amanzi e-BET.Ukutholwa kwe-endotoxin ngendlela ye-Kinetic Chromogenic kudinga isifundi se-microplate esifukamelayo esifana ne-ELx808IULAL-SN.

2. Ipharamitha yomkhiqizo

I-Assay Range: 0.005 - 50EU / ml;0.001 – 10EU/ml

3. Isici somkhiqizo kanye nesicelo

I-Bioendo^TMIkhithi Yokuhlola ye-KC Endotoxin (I-Kinetic Chromogenic Assay) ifaka ukumelana okuqinile nokuphazamiseka, futhi inezinzuzo ze-kinetic turbidimetric kanye nendlela ye-chromogenic yephoyinti lokugcina.Ilungele ukutholwa kwe-endotoxin yamasampula ebhayoloji njengomgomo, i-antibody, amaprotheni, i-nucleic acid, njll.

Qaphela:

I-Lyophilized Amebocyte Lysate reagent ekhiqizwa yi-Bioendo yenziwe nge-amebocyte lysate esuka kunkalankala yesicathulo samahhashi (i-Tachypleus tridentatus).

Isimo somkhiqizo:

Ukuzwela kwe-Lyophilized Amebocyte Lysate kanye namandla e-Control Standard Endotoxin kuhlolwa ngokumelene ne-USP Reference Standard Endotoxin.I-Lyophilized Amebocyte Lysate reagent kits iza nemiyalelo yomkhiqizo, Isitifiketi Sokuhlaziya.

Ikhithi yokuhlola ye-chromogenic endotoxin kufanele ikhethe isifundi se-microplate esinesihlungi esingu-405nm.

IUkuhlolwa kwe-Kinetic chromogenic lalisebenzisa ubuchwepheshe obusha be-chromogenic ukuze inikeze imiphumela enembile efika ku-0.005EU/ml, ikwenze kube ukukhetha okuhle kwalolo vivinyo lwezidakamizwa kumalabhoratri.Lokhu kuhlola kuklanyelwe ukuthola amazinga e-endotoxin kusampula anhlobonhlobo, okuhlanganisa imikhiqizo yemithi, izinto zezokwelapha, namasampuli emvelo.

Esinye sezici ezibalulekile zokuhlolwa kwe-KCA imvelo yayo ye-kinetic, evumela ukuqapha ngesikhathi sangempela amazinga e-endotoxin.Lokhu kusho ukuthi abasebenzisi bangakwazi ukulandelela ukuqhubeka kokuhlolwa njengoba kwenziwa, okuhlinzeka ngemininingwane ebalulekile ku-kinetics yokutholwa kwe-endotoxin.Le datha yesikhathi sangempela ingasiza abasebenzisi benze izinqumo ezinolwazi mayelana nekhwalithi nokuphepha kwemikhiqizo yabo, ekugcineni kuholele emiphumeleni ethuthukisiwe nokwaneliseka kwamakhasimende.

Ngaphezu kwalokho, i-ukuhlolwa kwe-chromogenic lalinikeza ukuzwela okungenakuqhathaniswa nokucacisa, iqinisekisa ukuthi ngisho namazinga aphansi e-endotoxin angabonakala ngokunembile futhi alinganiswe inani.Leli zinga eliphezulu lokunemba libalulekile ekuqinisekiseni ukuphepha nokusebenza ngempumelelo kwemikhiqizo yemithi kanye nemishini yezokwelapha, njengoba ukungcoliswa kwe-endotoxin kungase kube nomthelela omkhulu empilweni ezigulini nakubathengi.

Ngaphezu kwalokho, ukuhlolwa kwe-KCA kulula futhi kuyasebenziseka kalula, kudinga isikhathi esincane nokuqeqeshwa.Lokhu kuyenza ibe yisixazululo esikahle kumalabhorethri anesampula ephezulu noma izinsiza ezilinganiselwe, njengoba ingakwazi ukuhlehlisa inqubo yokuhlola i-endotoxin futhi ithuthukise ukusebenza kahle kwayo yonke.Idatha ye-KCAIsivivinyo se-LALingabuye ihlanganiswe kalula nokugeleza komsebenzi kwaselabhorethri okukhona, ukunciphisa ukuphazamiseka futhi yonge isikhathi esibalulekile nezinsiza.

Kafushane, iI-Kinetic Chromogenic LAL Endotoxin Test Assay(I-KCA) isivivinyo somthamo we-endotoxin esinikeza ukunemba okungenakuqhathaniswa, isivinini, kanye nokusebenziseka kalula.Ubuchwepheshe bayo obuyingqayizivele be-kinetic chromogenic namandla okuqapha ngesikhathi sangempela kuyenza ihluke ezindleleni zendabuko zokutholwa kwe-endotoxin, okuyenza ibe ukukhetha okuphezulu kubacwaningi, ochwepheshe bezokunakekelwa kwempilo, kanye nezinkampani zemithi.Ngokuhlolwa kwe-KCA, abasebenzisi bangaqinisekisa ngokuzethemba ukuphepha nekhwalithi yemikhiqizo yabo, okuholela emiphumeleni ethuthukisiwe nokwaneliseka kwamakhasimende.Izwa isizukulwane esilandelayo sokuhlolwa kwe-endotoxin ngokuhlolwa kwe-KCA.