Ukusetshenziswa kwe-Chromogenic Technique ekuhlolweni kwe-Bacterial Endotoxins

Indlela ye-Chromogenic iphakathi kwamasu amathathu aqukethe nendlela ye-gel-clot kanye nendlela ye-turbidimetric yokuthola noma ukulinganisa ama-endotoxins avela kubhaktheriya ye-Gram-negative ngokusebenzisa i-amoebocyte lysate ekhishwe egazini le-crab yehhashi eliluhlaza (i-Limulus polyphemus noma i-Tachypleus tridentatus).Ingahlukaniswa njenge-endpoint-chromogenic assay noma i-kinetic-chromogenic assay ngokusekelwe kumgomo othile wokuhlola osetshenzisiwe.

Isimiso sokusabela siwukuthi: i-amebocyte lysate iqukethe i-cascade yama-serine protease enzymes (ama-proenzymes) angasetshenziswa yi-endotoxin yebhaktheriya.Ama-endotoxins enza ama-proenzymes asebenze ukuze akhiqize ama-enzyme acushiwe (abizwa ngokuthi i-coagulase), lena yakamuva igqugquzela ukuhlukana kwe-substrate engenambala, ikhiphe umkhiqizo onombala ophuzi i-pNA.I-pNA ekhishiwe ingalinganiswa nge-photometrically ku-405nm.Futhi ukumuncwa kuhlotshaniswa kahle nokugxilwa kwe-endotoxin, khona-ke ukugxila kwe-endotoxin kungalinganiswa ngokufanele.