I-Chromogenic TAL Assay (i-Chromogenic endotoxin test assay)

I-Chromogenic TAL Assay (i-Chromogenic endotoxin test assay)

I-TAL reagent i-lyophilized amebocyte lysate ekhishwa egazini eliluhlaza okwesibhakabhaka le-Limulus polyphemus noma i-Tachypleus tridentatus.

Ama-Endotoxins ama-amphiphilic lipopolysaccharides (LPS) atholakala kulwelwesi lweseli olungaphandle lwebhaktheriya e-gram-negative.Imikhiqizo yabazali engcoliswe ama-pyrogens okuhlanganisa i-LPS ingaholela ekwakhiweni komkhuhlane, ukungeniswa kokusabela kokuvuvukala, ukushaqeka, ukwehluleka kwezitho kanye nokufa kumuntu.Ngakho-ke, amazwe emhlabeni jikelele enze imithethonqubo, edinga ukuthi noma yimuphi umkhiqizo womuthi othi awunyumba futhi awuna-pyrogenic kufanele uhlolwe ngaphambi kokukhululwa.Ukuhlolwa kwe-Gel-clot TAL kwaqalwa kwathuthukiswa ukuze kuhlolwe ama-endotoxins ebhaktheriya (okungukuthi, i-BET).Nokho, ezinye izindlela ezithuthuke kakhulu zokuhlolwa kwe-TAL sezivele.Futhi lezi zindlela ngeke zithole kuphela kodwa futhi zilinganise ubukhona be-endotoxins kusampula.

Ngaphandle kwesu le-gel-clot, amasu e-BET aqukethe nendlela ye-turbidimetric kanye nendlela ye-chromogenic.

I-Bioendo, enikezelwe ekutholeni i-endotoxin, ingumkhiqizi ochwepheshile wokuthuthukisa isivivinyo se-TAL se-chromogenic.I-Bioendo^TMIkhithi Yokuhlola ye-EC Endotoxin (I-End-point Chromogenic Assay) ihlinzeka ngokulinganisa okusheshayo komthamo we-endotoxin.Siphinde sihlinzeke nge-Bioendo^TMI-KC Endotoxin Test Kit (Kinetic Chromogenic Assay) kanye nesifundi se-incubation microplate ELx808IULALXH, esingaqinisekisa ukwethembeka nokusebenza kahle kokuhlolwa kwakho.