I-Chromogenic TAL Assay EC64405

I-TAL reagent, okungukuthi i-lyophilized amebocyte lysate ekhishwe egazini eliluhlaza lenkalankala yehhashi (i-Limulus polyphemus noma i-Tachypleus tridentatus), isetshenziswa njalo ukwenza ukuhlolwa kwe-bacterial endotoxins.

Kwa-Xieman Bioendo Technology Co., Ltd., sikhiqiza amakhithi okwenza uhlolo lwe-TAL lwephoyinti lokugcina lwe-chromogenic, oluhlanganisa zonke iziphehlisi ezidingekayo ku-BET ngoshintsho ekuthuthukisweni kombala, futhi sivumele ikhasimende ukuthi lithole imiphumela enembayo ngokushesha nje. isikhathi.

Izimiso zokutholwa kwe-chromogenic yephoyinti lokugcina nge-reagent ye-TAL yilezi: ukusabela kwe-enzymatic kwenzeka lapho i-endotoxin yebhaktheriya yenza i-factor C, futhi i-coagulase yakheka ngokufanele.Bese i-coagulase ihlukanisa i-substrate engenambala ukuze ikhiphe umkhiqizo onombala ophuzi i-pNA.I-pNA ekhishiwe ingalinganiswa nge-photometrically ku-405nm.Njengoba ukumuncwa kuhlotshaniswa kahle nokugxilwa kwe-endotoxin, ukugxila kwe-endotoxin kungase kulinganiswe ngokufanele.

I-EC64405, okungukuthi i-Bioendo^TMIkhithi Yokuhlola ye-EC Endotoxin (I-End-point Chromogenic Assay), iyikhithi yokuhlola ye-Chromogenic TAL engase isetshenziswe ukuze kutholwe inani le-endotoxin yebhaktheriya ekhona kusampula.Ikhithi ye-EC64405 ekhiqizwe yi-Bioendo ivumela ukuhlola okungu-64, futhi ukuzwela kuhlangana kusuka ku-0.1 EU/ml kuya ku-1 EU/ml.I-Bioendo's EC64405 iqukethe wonke ama-reagents adingekayo ukuze kuhlolwe i-chromogenic yephoyinti lokugcina.Njengoba futhi sinezinsiza ezingenayo i-endotoxin noma ezingenayo i-pyrogen, singaphinda sipakishe ikhithi ngokusekelwe kulokho okudingayo.

I-Incubating Microplate Reader iyadingeka ukuze kuhlolwe i-chromogenic iphuzu lokugcina.Isifundi sethu se-microplate esifukamelayo esingu-ELx808IULALXH sivumela amasampula ahlukene ukuthi atholwe ku-96-well microplate ngesikhathi esifanayo futhi sizohlaziya ukutholwa kwe-endotoxin ngokuzenzakalelayo nangokunembile.