Amashubhu engilazi ane-depyrogenation processing ayadingeka ekuhloleni i-endotoxin ukuze kuqinisekiswe ukunemba nokuthembeka kwemiphumela yokuhlolwa.Ama-endotoxins ayizingxenye zamangqamuzana azinzile odongeni lwamaseli angaphandle kwamanye amagciwane angenayo igram, futhi angabangela ukugula okukhulu ngisho nokufa kubantu uma ekhona emikhiqizweni yezokwelapha noma emishinini.
Ukuze kutholwe ama-endotoxins, i-assay isebenzisa ama-reagents aqukethe i-Limulus Amebocyte Lysate (LAL) noma abizwa nge-Lyophilized amebocyte lysate, ukukhishwa okuvela kumangqamuzana egazi enkalankala yehhashi enomshini wokujiyisa okwenziwa kusebenze ama-endotoxins.Kodwa-ke, amashubhu engilazi angazange akhishwe i-depyrogenated angaphazamisa i-assat yokuhlola ye-LAL ngokwenza kusebenze indlela yayo yokujiyisa futhi akhiqize imiphumela engelona iqiniso.Ngakho-ke, amashubhu engilazi asetshenziswa ekuhlolweni kwe-endotoxin kumele akhishwe ukuze kukhishwe noma yimaphi ama-endotoxin okungenzeka akhona futhi avimbele ukusebenza kwe-reagent ye-LAL.Lokhu kuqinisekisa ukuthi imiphumela yokuhlolwa kwe-endotoxin inembile futhi ithembekile nokuthi iziguli azichayeka emazingeni ayingozi e-endotoxin.futhi uqinisekise ukuphepha kwezidakamizwa zabazali kwezemithi, amaphrotheni, isiko lamaseli, iDNA nokunye.
Isidingo sokusebenzisa amashubhu engilazi angenayo i-endotoxin ekuhlolweni kokutholwa kwe-endotoxin:
Amashubhu engilazi angenayo i-endotoxinziyingxenye ebalulekile yanoma iyiphi i-endotoxin test assay.Lawa mashubhu engilazi aklanyelwe ukunciphisa ubungozi bokungcoliswa kwe-endotoxin phakathi nenqubo yokuhlola, ukuqinisekisa imiphumela enembile nethembekile.
Esinye sezici ezibalulekile zamashubhu engilazi angenayo i-endotoxin ukwakheka kwawo kwamakhemikhali.Lawa mashubhu enziwe ngengilazi ye-borosilicate yekhwalithi ephezulu, eyaziwa ngokumelana kwayo okukhethekile nokugqwala kwamakhemikhali.Lokhu kuzenza zilungele ukusetshenziswa ekuhlolweni kwe-endotoxin, njengoba zingakwazi ukumelana nokuchayeka ezinhlobonhlobo zezinhlanganisela zokuhlola ngaphandle kokululaza noma ukungcolisa isampula.
Esinye isici esibalulekile samashubhu engilazi angenayo i-endotoxin ukuhlanzeka kwawo.Lawa mashubhu ahlanzwa ngokucophelela futhi ahlinzwe inzalo ngaphambi kokusetshenziswa ukuze kususwe noma yimiphi imithombo engaba khona yokungcola.Ziphinde zihlolwe ngokuqinile ukuthi zingcolile yini i-endotoxin, kuqinisekwe ukuthi azinawo wonke amanani ale nto eyingozi.
Ngaphezu kwalokho, amashubhu engilazi angenayo i-endotoxin aklanyelwe ukuthi abe lula ukuwasebenzisa.Ngokuvamile zitholakala ngebanga losayizi nokucushwa ukuze kufakwe amavolumu esampula ahlukene nezindlela zokuhlola kokubili ukuhlolwa kwekhwalithi ye-endotoxin kanye nenani lokuhlola i-endotoxin.Zibuye zihambisane nezinhlobonhlobo zesampula yokulungiselela kanye nemishini yokuhlola, okuyenza ibe yinketho eguquguqukayo futhi elula yamalabhorethri okuhlola i-endotoxin.
Sekukonke, amashubhu engilazi angenayo i-endotoxin adlala indima ebalulekile ekunembeni nasekuthembekeni kokuhlolwa kwe-endotoxin.Ukwakhiwa kwazo kwekhwalithi ephezulu, ubumsulwa, kanye nokusebenziseka kalula kwazo kuzenza zibe ingxenye ebalulekile yanoma ikuphi ukuhlolwa okuphumelelayo kwe-endotoxin.
Amashubhu engilazi angenayo i-Bioendo Endotoxin anosayizi10*75mm, 12*75mm, 13*100mm kanye 16*100mmngezinqubo zokuhlanjululwa kanye nezinqubo zokusabela.
Amashubhu engilazi angenayo i-endotoxin ahlangabezana nezinga eliphezulu lezinga le-endotoxins elingaphansi kuka-0.005EU/ml.
Amashubhu engilazi angenayo i-endotoxin kufanele asetshenziswe ekuhlolweni kwe-gel clot endotoxin ukuvimbela imiphumela engemihle.
Ama-endotoxins ayizingxenye zodonga lwamaseli ebhaktheriya ezingangcolisa impahla yaselabhorethri, okuhlanganisa namashubhu engilazi.
Ukuhlolwa kwe-clot endotoxin yejeli kusetshenziselwa ukuthola ubukhona be-endotoxin kusampula.Kulesi sivivinyo, kwakheka ihlule phambi kwama-endotoxins.Lokhu kwakheka kwehlule bese kuqhathaniswa nesilawuli sokunquma ukugxiliswa kwe-endotoxin.
Ukusebenzisa amashubhu engilazi angenayo i-endotoxin kusiza ukuqinisekisa ukuthi ukutholwa kwe-endotoxin kunembile.Lokhu kungenxa yokuthi ama-endotoxins angakwazi ukunamathela ebusweni bamashubhu engilazi futhi aphazamise imiphumela yokuhlola.
Ukuqinisekisa ukuthi amashubhu engilazi asetshenziswa ekuhlolweni kwe-gel clot endotoxin test angenayo i-endotoxin, kufanele awashwe ngomuthi wokuhlanza bese ehashwa kahle ngamanzi angenayo i-endotoxin.Ukwengeza, kufanele zihlanzwe kusetshenziswa i-autoclaving noma inzalo yokushisa eyomile.
Sengiphetha, ukusebenzisa amashubhu engilazi angenayo i-endotoxin ekuhlolweni kokuhlolwa kwe-clot endotoxin yejeli kubalulekile ukuze kuqinisekiswe ukutholwa okunembile kwama-endotoxin.Lawa mashubhu kufanele ahlanzwe kahle futhi abulawe amagciwane ukuze kuqedwe noma yikuphi ukungcola okungase kube khona.
Isikhathi sokuthumela: Jun-02-2023
