Amanzi angenayo i-Endotoxin adlala indima ebalulekile ekunembeni nasekuthembekeni komsebenzi wokuhlola i-endotoxin.I-Endotoxins, eyaziwa nangokuthi i-lipopolysaccharides (LPS), ayizinto ezinobuthi ezitholakala ezindongeni zamaseli we-Gram-negative bacteria.Lokhu kungcola kungabangela ukulimala okukhulu kubantu nasezilwaneni uma kungasuswa emikhiqizweni yezokwelapha njengemithi yokugoma, izidakamizwa, kanye nemishini yezokwelapha.
Ukuze kutholwe futhi kulinganise amazinga e-endotoxin ngokunembile, ukuhlolwa kwe-endotoxin kuncike ekuhlolweni okubucayi okudinga ukusetshenziswa kwamanzi angenayo i-endotoxin.Lolu hlobo lwamanzi luphathwa ukuze lususe yonke iminonjana ye-endotoxins, kuqinisekiswe ukuthi noma yimiphi imiphumela emihle ekhiqizwe i-assay ibangelwa kuphela ukuba khona kwe-endotoxin kusampula ehlolwayo, futhi hhayi umphumela wokungcoliswa kwamanzi.
Ukusebenzisa amanzi angenayo i-endotoxin kusiza futhi ekwehliseni imiphumela emihle engamanga, engenzeka uma kukhona umkhondo wamanani e-endotoxin emanzini asetshenziswe ekuhlolweni.Lokhu kungaholela emiphumeleni engalungile, engase ibangele ukubambezeleka ekukhishweni komkhiqizo nezinkinga zokulawula.
Kafushane, amanzi angenayo i-endotoxin ayingxenye ebalulekile yokusebenza kokuhlolwa kwe-endotoxin, okuqinisekisa ukunemba nokuthembeka kwalokhu kuhlolwa okubucayi.Ngokunciphisa ubungozi bemiphumela engamanga nokuqinisekisa ukuthi imiphumela emihle ikhiqizwa kuphela lapho kukhona ukungcoliswa kwe-endotoxin kwangempela, amanzi angenayo i-endotoxin adlala indima ebalulekile ekuqinisekiseni ukuthi imikhiqizo yezokwelapha iphephile futhi iyasebenza ukuze isetshenziswe ezigulini.
Amanzi okuhlola i-bacterial endotoxin
Umehluko phakathi kwamanzi okuhlola i-endotoxin yebhaktheriya namanzi angenalutho omjovo: i-pH, i-endotoxin yebhaktheriya kanye nezici zokuphazamiseka.
Amanzi okuhlola i-bacterial endotoxin
Umehluko phakathi kwamanzi okuhlola i-endotoxin yebhaktheriya namanzi angenalutho omjovo: i-pH, i-endotoxin yebhaktheriya kanye nezici zokuphazamiseka.
1. pH
I-pH efaneleke kakhulu yokusabela phakathiI-reagent ye-LALfuthi i-endotoxin ingu-6.5-8.0.Ngakho-ke, ekuhlolweni kwe-LAL, i-United States, i-Japan Pharmacopoeia kanye noshicilelo lwango-2015 lwe-Chinese Pharmacopoeia lubeka ukuthi inani le-pH lomkhiqizo wokuhlola kufanele lilungiswe libe ngu-6.0-8.0.Inani le-pH lamanzi lokuhlolwa kwe-endotoxin yebhaktheriya ngokuvamile lilawulwa ku-5.0-7.0;inani le-pH lamanzi oyinyumba omjovo kufanele lilawulwe ku-5.0-7.0.Njengoba imithi eminingi ine-asidi ebuthakathaka, inani le-pH lamanzi lokuhlolwa kwe-endotoxin yebhaktheriya lilungele ukuhlolwa kwe-endotoxin noma ukuhlolwa kwe-Lyophilized amebocyte lysate test.
2. I-Endotoxin yegciwane
Inani le-endotoxin emanzini ukuze kuhlolwe i-endotoxin yebhaktheriya kufanele okungenani libe ngaphansi kuka-0.015EU nge-1ml ngayinye, futhi inani le-endotoxin emanzini ukuze kuhlolwe i-endotoxin yebhaktheriya ngezindlela zobuningi kufanele libe ngaphansi kuka-0.005EU nge-1ml ngayinye;Amanzi angenalutho omjovo kufanele aqukathe ngaphansi kuka-0.25 EU we-endotoxin nge-1ml ngayinye.
I-endotoxin emanzini yokuhlolwa kwe-endotoxin yebhaktheriya kufanele ibe phansi ngokwanele ukuthi akufanele ithinte imiphumela yokuhlolwa.Uma kusetshenziswa amanzi angenalutho omjovo esikhundleni samanzi okuhlola okuhlolwa kwe-Endotoxin, ngenxa yokuqukethwe okuphezulu kwe-endotoxin emanzini angenalutho ukuze umjovo, amanzi angenalutho omjovo kanye Nokubekwa okuphezulu kwe-endotoxin kusampula ehloliwe kungase kukhiqize amanga, okubangela ukulahlekelwa okuqondile kwezomnotho. ebhizinisini.Ngenxa yomehluko wokuqukethwe kwe-endotoxin, akwenzeki ukuthi kusetshenziswe amanzi angenalutho ukuze umjovo esikhundleni samanzi okuhlola ukuze kuhlolwe i-endotoxin assay noma i-Lyophilized amebocyte lysate test assay.
3. Izinto Eziphazamisayo
Amanzi okuhlolwa kwe-endotoxin yebhaktheriya akumele aphazamise i-reagent ye-LAL, alawule i-endotoxin evamile kanye nokuhlolwa kwe-LAL;asikho isidingo samanzi oyinyumba ngomjovo.Amanzi angenalutho omjovo adinga ukuphepha nokuzinza, kodwa ingabe amanzi angenalutho omjovo azowuthinta umsebenzi nokuzinza kwe-endotoxin evamile yokulawula amagciwane?Ingabe Amanzi Ayinyumba Omjovo Ayathuthukisa Noma Avimbele Ukuhlolwa Kwe-endotoxin?Bambalwa abantu abenze ucwaningo lwesikhathi eside ngalokhu.Kuye kwaqinisekiswa ngophenyo ukuthi amanye amanzi angenalutho omjovo anomphumela oqinile wokuvinjelwa ekuhlolweni kwe-LAL.Uma kusetshenziswa amanzi angenalutho omjovo esikhundleni samanzi okuhlola okuhlolwa kwe-LAL, kungase kube negethivu engelona iqiniso, okuholela ekuphuthelweni kokutholwa kwe-endotoxin, okusongela ukuphepha komuthi ngokuqondile.Ngenxa yokuba khona kwezinto eziphazamisayo zamanzi angenalutho ukuze umjovo, akwenzeki ukuthi kusetshenziswe amanzi angenalutho ukuze umjovo esikhundleni samanzi okuhlola ukuhlolwa kwe-LAL.
Uma ukunemba kwamanzi okugeza, indlela yokugeza kanye namanzi okuhlola kungaqinisekiswa, ukuthi kungenzeka ukuthi ukulawulwa okuhle ekuhlolweni kwe-Limulus akukwazi ukusungulwa ngokuyisisekelo akukho, ngaphandle uma izinga elisetshenzisiwe lingalingani.Ukuze siqinisekise ukunemba kwemiphumela yokuhlolwa, kufanele:
a.Ukujwayelana namazinga nezinkambiso zemboni;
b.Sebenzisa imikhiqizo efanelekile kanye nemikhiqizo ejwayelekile;
c.Sebenza ngokuhambisana ngokuqinile nezinqubo zokusebenza.
Isikhathi sokuthumela: Jul-26-2023
