Amanzi Angenayo I-Endotoxinvs Amanzi Ahlanzekile: Ukuqonda Umehluko Obalulekile
Emhlabeni wocwaningo nokukhiqizwa kwelabhorethri, amanzi adlala indima ebalulekile ekusetshenzisweni okuhlukahlukene.Izinhlobo ezimbili zamanzi ezivame ukusetshenziswa kulezi zilungiselelo amanzi angenayo i-endotoxin kanye namanzi ahlanzekile.Nakuba lezi zinhlobo ezimbili zamanzi zingase zibonakale zifana, azifani.Eqinisweni, kukhona umehluko oyinhloko phakathi kokubili okubalulekile ukuqonda ukuze kuqinisekiswe impumelelo nokunemba kwemiphumela yokuhlola.
Kulesi sihloko, sizohlola umehluko phakathi kwamanzi angenayo i-endotoxin namanzi ahlanzekile, futhi sixoxe ngokusetshenziswa kwawo nokubaluleka kwawo endaweni yaselabhorethri.
Amanzi angenayo i-endotoxin amanzi ahlolwe kahle futhi aqinisekiswa ukuthi awanawo ama-endotoxins.Ama-endotoxins ayizinto ezinobuthi ezikhishwa ezindongeni zamangqamuzana amagciwane athile, futhi angabangela uhla lwemiphumela emibi ezinhlelweni zebhayoloji, okuhlanganisa ukuvuvukala kanye nokwenza kusebenze ukuphendula kwamasosha omzimba.Ngokuphambene, amanzi e-ultrapure asho amanzi ahlanzwe ngezinga eliphezulu kakhulu ngangokunokwenzeka, ngokuvamile ngezinqubo ezifana ne-osmosis ehlehlayo, i-deionization, ne-distillation, ukuze kukhishwe ukungcola okufana nama-ion, ama-organic compounds, nama-particle.
Omunye umehluko oyinhloko phakathi kwamanzi angenayo i-endotoxin namanzi e-ultrapure usezinkambisweni zawo zokuhlanza.Nakuba amanzi ahlanzekile ethola ukwelashwa okuqinile ngokomzimba namakhemikhali ukuze asuse ukungcola ezingeni lamangqamuzana, amanzi angenayo i-endotoxin agxile ngokukhethekile ekukhipheni ubuthi be-endotoxin ngokusebenzisa ukuhlunga okukhethekile nezindlela zokuhlanza.Lo mehluko ubalulekile ngoba nakuba amanye ama-endotoxins angase asuswe ngempumelelo ngezinqubo zokuhlanza amanzi e-ultrapure, asikho isiqinisekiso sokuthi wonke ama-endotoxin azoqedwa ngaphandle kokwelashwa okuqondile kwamanzi angenayo i-endotoxin.
Omunye umehluko obalulekile phakathi kwezinhlobo ezimbili zamanzi ukusetshenziswa kwawo okuhloswe ngawo elabhorethri kanye nezilungiselelo zokukhiqiza.Amanzi ahlanzekile avame ukusetshenziswa ezinhlelweni lapho ukungabi bikho kokungcola ezingeni lamangqamuzana kubaluleke kakhulu, njengokulungiswa kwama-reagents, ama-buffers, kanye nemidiya yesiko lamaseli nokuhlolwa kwebhayoloji yamangqamuzana.Ngakolunye uhlangothi, amanzi angenayo i-endotoxin aklanyelwe ukusetshenziswa ekuhloleni nasezinqubweni lapho ubukhona be-endotoxin bungase bubeke engcupheni ukunemba nokuthembeka kwemiphumela.Lokhu kufaka phakathi izinhlelo zokusebenza ezifana nezifundo ze-in vitro ne-in vivo, ukukhiqizwa kwemithi, nokukhiqizwa kwemishini yezokwelapha, lapho umthelela ongaba khona wama-endotoxin kumasistimu weselula nawebhayoloji kufanele uncishiswe.
Kuyaphawuleka ukuthi nakuba amanzi angenayo i-endotoxin kanye namanzi e-ultrapure esebenza ngezinhloso ezahlukene, awahlukani.Eqinisweni, ezindaweni eziningi zaselabhorethri nezokukhiqiza, abacwaningi nososayensi bangasebenzisa zombili izinhlobo zamanzi kuye ngezidingo ezithile zokuhlola nezinqubo zabo.Isibonelo, lapho kukhiqizwa amaseli elabhorethri, amanzi e-ultrapure angase asetshenziselwe ukulungisa imidiya ye-cell culture kanye nama-reagents, kuyilapho amanzi angenayo i-endotoxin angase asetshenziswe ekugezeni kokugcina nasekulungiseni izindawo zamaseli ukuze kuqinisekiswe ukungabikho kwama-endotoxins angaphazamisa. imiphumela yokuhlola.
Sengiphetha, kubalulekile ukukuqaphela lokhoamanzi angenayo i-endotoxinfuthi amanzi ahlanzekile ayizinhlobo ezihlukile zamanzi asebenza ngezinhloso ezehlukene elabhorethri kanye nezilungiselelo zokukhiqiza.Ukuqonda umehluko phakathi kwalokhu okubili, okuhlanganisa izinqubo zokuhlanzwa kanye nokusetshenziswa okuhlosiwe, kubalulekile ukuze kuqinisekiswe ukunemba nokuthembeka kwemiphumela yokuhlola.Ngokusebenzisa uhlobo olufanele lwamanzi kuhlelo ngalunye, abacwaningi nososayensi banganciphisa ubungozi bokungcola nokuhlanekezela emsebenzini wabo, ekugcineni kube nesandla ekuthuthukisweni kolwazi lwesayensi nokusungula izinto ezintsha.
Isikhathi sokuthumela: Dec-06-2023
