Ukuhlolwa kwe-bacterial endotoxin (BET) kwenziwa kumalabhorethri amaningi esimanje ngaphansi kwezimo ezilawulwayo njengento ebalulekile yokugwema ukuphazamiseka.
Kuyafanelekainqubo ye-aseptickubalulekile lapho ulungiselela futhi uhlanza amazinga kanye namasampula okuphatha.Ukugqokaukuzijwayeza ngaphandle kwemishini yokuzivikela yaselabhorethri evamile (I-PPEIzimfuneko aziyona into ekhathazayo ngaphandle uma umkhiqizo ongaphansi kokuhlolwa ufuna ukucatshangelwa okukhethekile kokuphepha komhlaziyi ngenxa yobuthi noma ukusuleleka.Amagilavukufanele ingabi ne-TALC, njengoba i-TALC ingase ibe namazinga abalulekile we-endotoxins.Izifundi zepuleti, okugeza amanzi, kanye namabhlogo okushisa omileesetshenziselwa ukufukamela isampula kufanele kube sebhentshini laselabhorethri eliqhelelene nemigudu yokushisisa, yokukhipha umoya, kanye neye-air conditioning (HVAC), ukudlidliza okubalulekile, kanye nokuhamba kwelabhorethri okungase kuthinte imiphumela yokuhlolwa.Izikhathi zokubamba amasampula nezimokufanele kunqunywe futhi kamuva kubhalwe phansi, uma kunesidingo, ukuze kuqinisekiswe ukuthi imiphumela yokuhlolwa enembile ingakhiqizwa ngesikhathi esifanele.
Isibonelo, uma ilabhorethri ithola Amanzi Omjovo (i-WFI) noma isampula elisacutshungulwa, kufanele ifakwe esiqandisini noma ingahlala ezingeni lokushisa elilingana negumbi, futhi isikhathi esingakanani?Ngaphambi kokuhlolwa, kunconywa ukuthi i(izi)qukathi eziyisisekelo zesampula zixutshwe ngokwanele ngaphambi kokukhipha ama-aliquot wokuhlola ukuze kuhlolwe okuqondile noma ukuhlanjululwa okulandelayo.
Ukuhlolwa kwe-Bioendo Bacterial Endotoxin, ukuhlolwa kuhlanganisaindlela ye-gel clotukuhlolwa kwe-endotoxin kanyei-quantitative endotoxin test assay, indlela yejeli yehlule le-endotoxin yokuhlola iwukutholwa kwe-endotoxin esezingeni, lokhu kuhlola kudinga okusebenzisekayo ukucutshungulwa kwe-depyrogenation, njengamashubhu okusabela angenayo i-endotoxin, amashubhu okuhlanjululwa kanye namathiphu angenayo i-pyrogen;ukutholwa kwe-endotoxin yobuningi kunokuhlolwa kwe-kinetic chromogenic endotoxin, ukuhlolwa kwe-endotoxin ye-kinetic turbidimetric, lokhu kuhlola kudinga okusebenzisekayo kufanele kuhlangabezane nezinga eliphezulu le-endotoxins ngaphansi kwe0.005EU/ml( 0.001EU/ml), njengamashubhu angenayo i-endotoxin, amathiphu angenayo i-pyrogen, nama-microplates angenayo i-pyrogen, ngisho ne-pyrogen free revoir.Kodwa-ke, uma ukwelashwa kwamasampula, isitsha kufanele kube ibhodlela lesampula elingenayo i-endotoxin.
Ekuhlolweni kwe-endotoxin, ukuphazamiseka kungavela emithonjeni eyahlukene, njengesampula yezingxenye ze-matrix, ama-reagents okuhlola, noma okokusebenza.
Ukuze kugwenywe ukuphazamiseka kokuhlolwa, izinyathelo ezilandelayo zingathathwa:
1. Ukulungiselela Isampula: Ukulungiswa kwesampula okufanele kubalulekile ekuhlolweni okunembile kwe-endotoxin.
Isampula ye-matrix kufanele ihlolwe ngokuphelele futhi ithuthukiswe ukuze kuqinisekiswe ukuhambisana ne-endotoxin assay.
Ikakhulukazi, izinto eziphazamisayo ezifana nama-lipids namaprotheni kufanele zisuswe noma zincishiswe kusetshenziswa amasu afanelekile afana nokuhlunga noma ukugxilisa ingqondo.
2. Ukulawula Okuhle Nokubi: Kubalulekile ukufaka izilawuli ezinhle nezingezinhle ekuhlolweni ukuze kuqashwe ukuphazanyiswa.
Izilawuli ezinhle ziqinisekisa ukusebenza kokuhlolwa, kuyilapho izilawuli ezingezinhle zithola noma yikuphi ukungcoliswa noma ukuphazamiseka okuvela ezingxenyeni zokuhlola.
3. Ukulawulwa Kwekhwalithi: Ukulawulwa kwekhwalithi kufanele kwenziwe kuwo wonke ama-reagents, okokusebenza, namanzi asetshenziswa ekuhlolweni.
Lokhu kuqinisekisa ukuthi ama-reagents awangcoliswa yi-endotoxin futhi asebenza ngendlela efanele.
4. Ukulinganisa: Ukuhlolwa kufanele kufane ukuze kuqinisekiswe ukuthi yonke imiphumela iyaqhathaniseka futhi iphinde ikhiqizeke.
Lokhu kuhilela ukusetshenziswa kwejika elijwayelekile ukuze kulinganiswe isivivinyo kanye nokusetshenziswa kwamasu amisiwe okulungiselela isampula, ukufukamela, nokutholwa.
5. Ukuqinisekisa: Ukuhlolwa kufanele kuqinisekiswe ukuze kuqinisekiswe ukuthi kucacile, kubucayi, futhi kuthembekile.
Lokhu kuhilela ukuhlola uhla lwamasampuli, okuhlanganisa nalawo aziwayo ukuthi aqukethe i-endotoxin, ukuze kutholakale ukunemba nokunemba kokuhlolwa.
Ngokulandela lezi zinyathelo, ukuphazamiseka kungancishiswa, futhi ukuhlolwa okunembile kwe-endotoxin kungafinyelelwa.
Isikhathi sokuthumela: Dec-01-2022
