I-Kinetic Chromogenic Endotoxin Test Assay (i-Chromogenic LAL/TAL assay)

I-KCET- Kinetic Chromogenic Endotoxin Test Assay (Isivivinyo sokuhlola i-Chromogenic endotoxin siyindlela ebalulekile yamasampuli anokuphazamiseka okuthile.)
Ukuhlolwa kwe-kinetic chromogenic endotoxin (KCT noma i-KCET) kuyindlela esetshenziswa ukuthola ubukhona be-endotoxin kusampula.
I-Endotoxins yizinto ezinobuthi ezitholakala ezindongeni zeseli zezinhlobo ezithile zamagciwane, okuhlanganisa amagciwane ane-gram-negative afana ne-Escherichia coli ne-Salmonella.Esivivinyweni se-KCET, i-substrate ye-chromogenic yengezwa kusampula, esabela nanoma yimaphi ama-endotoxin akhona ukukhiqiza ushintsho lombala.
Izinga lokuthuthukiswa kombala ligadwa ngokuhamba kwesikhathi kusetshenziswa i-spectrophotometer, futhi inani le-endotoxin kusampula libalwa ngokusekelwe kuleli zinga.
Ukuhlolwa kwe-KCT kuyindlela edumile yokuthola ama-endotoxins kwezemithi, izinto zezokwelapha, neminye imikhiqizo ehlangana nomzimba womuntu.Isivivinyo esibucayi futhi esithembekile esikwazi ukubona ngisho nenani elincane kakhulu le-endotoxin, okuyenza ibe ithuluzi elibalulekile lokuqinisekisa ukuphepha kwale mikhiqizo.

 

I-TAL/LAL reagent i-lyophilized amebocyte lysate ekhishwa egazini eliluhlaza okwesibhakabhaka le-Limulus polyphemus noma i-Tachypleus tridentatus.

Ama-Endotoxins ama-amphiphilic lipopolysaccharides (LPS) atholakala kulwelwesi lweseli olungaphandle lwebhaktheriya e-gram-negative.Imikhiqizo yabazali engcoliswe ama-pyrogens okuhlanganisa i-LPS ingaholela ekwakhiweni komkhuhlane, ukungeniswa kokusabela kokuvuvukala, ukushaqeka, ukwehluleka kwezitho kanye nokufa kumuntu.
Ngakho-ke, amazwe emhlabeni jikelele enze imithethonqubo, edinga ukuthi noma yimuphi umkhiqizo womuthi othi awunyumba futhi awuna-pyrogenic kufanele uhlolwe ngaphambi kokukhululwa.Ukuhlolwa kwe-Gel-clot TAL kwaqalwa kwathuthukiswa ukuze kuhlolwe ama-endotoxins ebhaktheriya (okungukuthi, i-BET).
Nokho, ezinye izindlela ezithuthuke kakhulu zokuhlolwa kwe-TAL sezivele.Futhi lezi zindlela ngeke zithole kuphela kodwa futhi zilinganise ubukhona be-endotoxins kusampula.Ngaphandle kwesu le-gel-clot, amasu e-BET aqukethe nendlela ye-turbidimetric kanye nendlela ye-chromogenic.I-Bioendo, enikelwe ekutholeni i-endotoxin, ingumkhiqizi ochwepheshile wokuthuthukisa i-chromogenic TAL/LAL assay.
Ikhithi Yokuhlola I-Bioendo EC Endotoxin (I-End-point Chromogenic Assay) inikeza ukulinganisa okusheshayo komthamo we-endotoxin.
Siphinde futhi sinikeze i-Bioendo KC Endotoxin Test Kit (Kinetic Chromogenic Assay) kanye nesifundi se-incubation microplate ELx808IU-SN, esingaqinisekisa ukwethembeka nokusebenza kahle kokuhlolwa kwakho.
Yiziphi izici ze-i-kinetic chromogenic endotoxin test assayukuhlola ama-endotoxins kumasampuli?

I-kinetic chromogenic endotoxin test assay ingenye indlela esetshenziselwa ukuhlola ama-endotoxins kumasampuli.Inezici ezimbalwa:
1. Ukulinganisa kwe-kinetic: Ngokufanayo nokuhlolwa kwe-turbidimetric, ukuhlolwa kwe-chromogenic kinetic kuhlanganisa nokulinganisa kwe-kinetic.Incike ekuphenduleni phakathi kwe-endotoxins ne-chromogenic substrate ukukhiqiza umkhiqizo onemibala.Ukushintsha kokuqina kombala ngokuhamba kwesikhathi kuyagadwa, okuvumela ukulinganisa kokugxila kwe-endotoxin kusampula.
2. Ukuzwela okuphezulu: I-kinetic chromogenic assay izwela kakhulu futhi ingathola amazinga aphansi we-endotoxin kumasampuli.Ingakwazi ukukala ngokunembile ukugxila kwe-endotoxin, ngisho nasemazingeni aphansi kakhulu, iqinisekise ukutholwa nokulinganisa okuthembekile.
3. Ububanzi obuguquguqukayo obubanzi: I-assay inobubanzi obuguquguqukayo obubanzi, okuvumela ukulinganiswa kokugxiliswa kwe-endotoxin ku-spectrum ebanzi.Lokhu kusho ukuthi ingahlola amasampula anamazinga ahlukahlukene e-endotoxins, afaka kokubili ukugxila okuphansi nokuphezulu ngaphandle kwesidingo sokuhlanjululwa kwesampula noma ukugxilisa ingqondo.
4. Imiphumela esheshayo: I-kinetic chromogenic assay inikeza imiphumela esheshayo uma iqhathaniswa nezindlela zendabuko.Ngokuvamile inesikhathi esifushane sokuhlola, esivumela ukuhlola okusheshayo nokuhlaziywa kwamasampuli.Ukuthuthukiswa kombala kungagadwa ngesikhathi sangempela, futhi imiphumela ngokuvamile ingatholwa phakathi namaminithi ambalwa kuya emahoreni ambalwa, kuye ngekhithi ethile yokuhlola nezinto ezisetshenziswayo.
5. Ukuzenzakalela kanye nokumiswa: Ukuhlolwa kungenziwa kusetshenziswa amasistimu azenzakalelayo, njengezifundi ze-microplate noma
abahlaziyi be-endotoxin-specific.Lokhu kuvumela ukuhlolwa komphumela ophezulu futhi kuqinisekisa izilinganiso ezingaguquki nezijwayelekile, kunciphisa amaphutha omuntu nokwandisa ukusebenza kahle.
6. Ukuhambisana nezinhlobo zamasampula ahlukahlukene: Ukuhlolwa kwe-chromogenic kwe-kinetic kuhambisana nohlu olubanzi lwezinhlobo zesampula, okuhlanganisa imithi, imishini yezokwelapha, i-biologics, namasampula amanzi.Kuyindlela eguquguqukayo engasetshenziswa ezimbonini nasezinhlelweni ezahlukene lapho kudingeka khona ukuhlolwa kwe-endotoxin.

 

Sekukonke, i-kinetic chromogenic endotoxin test assay inikeza indlela ebucayi, esheshayo, nethembekile yokuthola kanye nokulinganisa
ama-endotoxins kumasampula.Isetshenziswa kakhulu embonini yezemithi, i-biotechnology, kanye nezimboni zokunakekelwa kwezempilo ukulawula ikhwalithi nokuphepha.
izinjongo zokuhlola.