I-Endotoxin Test Assay eyenziwe yi-Lyophilized Amebocyte Lysate (LAL Reagent)

I-Endotoxin Test Assay eyenziwe yi-Lyophilized Amebocyte Lysate (LAL Reagent)

Ama-reagents e-LAL: I-Lyophilized amebocyte lysate (LAL) iyi-aqueous extract yamangqamuzana egazi (amebocyte) asuka ku-Atlantic horseshoe crab.
I-TAL Reagents: I-TAL reagent iwukukhipha okunamanzi kwamangqamuzana egazi asuka ku-Tachypleus tridentatus.
Njengamanje, ukukhiqizwa okuyinhloko kwama-reagents e-LAL/TAL ase-United States nase-China.

Ukuhlolwa kwe-gel clot endotoxin test assay, le ndlela kuseyiyona ndlela esetshenziswa kakhulu emhlabeni wonke, ukuze kuhlangatshezwane nezidingo zokuphepha zemithi yomjovo womuntu.
OKWAMANJE i-Bioendo ikhiqiza futhi inikeze i-gel clot reagent ye-LAL kuhlanganise nama-ampule engilazi yokuhlola eyodwa kanye nezitsha zokuhlola eziningi.
https://www.bioendo.com/gel-clot-endotoxin-assay/ G01, GS44, G02, G17 kanye ne-G52
Lesi yisixazululo esinomnotho sokutholwa kwe-endotoxin esezingeni ekuhlolweni komuthi.Ikakhulukazi izidakamizwa ezijovwayo noma izidakamizwa zomzali ukuhlola ama-endotoxins ku-WFI, i-API noma imikhiqizo yezidakamizwa eqediwe.Ukuhlolwa kokuhlolwa kwe-Endotoxin kuyisidingo esikhulu sezenzo zokusebenza, kudinga u-opharetha onekhono ukuze abhekane nokuhlolwa kwehlule lejeli ukuze kuqinisekiswe imiphumela efanele.

Isixazululo esiphelele seG52Ukuhlolwa kwe-Endotoxin:
I-LAL Reagent
Lawula i-Endotoxin Ejwayelekile
BET Amanzi
Amathiphu e-pipette angenayo i-Endotoxin
Amashubhu engilazi angenayo i-endotoxin, okuhlanganisa ukusebenza kokuhlanjululwa kanye namashubhu okusabela.
Ithuluzi lokufukamela, ukuncoma ukugeza kwamanzi noma i-incubator yokushisa eyomile.Wonke amadivaysi e-incubation adinga ukunemba kwezinga lokushisa.
Konke okusebenzisekayo ukuze uthinte i-LAL assay efinyelela izinga le-endotoxin-free level ” < 0.005EU/ml ” .
Indawo yokuhlola izohambisana nokutholwa kwe-endotoxin.

Naka:
Izinto ezisetshenziswa epulasitiki njengamathiphu e-pipette noma amapuleti e-multiwell asetshenziswa kakhulu ekuhlolweni kwe-endotoxin.I-Pharmacopoeias (USP/CP) evumelanisiwe idinga ukuthi noma yiziphi izinto zepulasitiki ezidlekayo namashubhu engilazi kufanele kungabi ne-endotoxin ebonakalayo futhi kubaluleke ngokulinganayo kodwa ngokuvamile kungacatshangwa, kufanele kube izinga lokungabi nazici eziphazamisayo.

Isetshenziswa kanjani i-endotoxin test assay ukuthola ama-endotoxins kusampula?
Okokuqala, ukuhlolwa kokuqinisekiswa kokuzwela kwe-lysate kufanele kwenziwe.Ukuqinisekisa ukuzwela kuyefana kumalebula.
Ukuze uthole ukuhlaziywa kwesampula, yenza ukuhlolwa kokuhlolwa kwe-endotoxin kwangaphambilini.
Ukuze usebenzise i-endotoxin assay ephelele "yokuhlolwa kokuphazanyiswa".
Ukuze usebenzise ukuhlolwa komkhawulo ukuze uthole ukuthi injani ileveli ye-endotoxins kusampula.

Lapho kwenziwa ukuhlolwa kokuqinisekiswa kokuzwela kwe-lysate, umphumela awujwayelekile, i-2 Lamada havenot gel formation?
Hlola ukuthi indlela yokulungiselela ye-endotoxin evamile yokulawula amagciwane ilungile yini.
Ukuxutshwa kwe-Vortex kuyadingeka ku-diluent ngayinye (bona imanuwali yeziqondiso noma okubizwa ngokuthi ukufaka kwe-endotoxin evamile yokulawula ngemininingwane).
Ukuhlola ukuthi i-reagent ye-lysate ifana yini ne-endotoxin evamile yokulawula.

Kunconywa ukusebenzisa ukugeza kwamanzi noma i-incubator yokushisa eyomile yenqubo yokushisa eqhubekayo ye-lysate reagent (isibonelo, i-incubator noma ibhokisi elomile alisebenzi).
Ngesikhathi sokufukamela, imvelo yamanzi emanzini okugeza amile futhi azinzile, konke ukudlidliza kuyagwenywa.
Naka ngokukhethekile ukugeza kwamanzi ukuze uvale iphampu yokugeleza kwamanzi.

Uma ubheka umphumela, khipha ngobumnene ishubhu lokuhlola noma i-ampoule yengilazi ku-thermostat, guqula kancane kancane ama-degree angu-180,
ukwakheka kwejeli ku-tube ngeke kukhubazeke, futhi ngeke kushelele, umphumela ubhalwe ngesignali "+";
akukho ukwakheka kwejeli okwakhiwa noma ihlule lejeli alikwazi ukugcinwa liqinile nakuba lakhiwe.
I-wall slip inegethivu, umphumela uqoshwe ngesignali "-".
Ikhithi yokuhlola ihlule lejeli elisheshayo iyingxenye yendlela yokuhlola ihlule lejeli endotoxin.
Ekuhlolweni kwe-endotoxin ye-gel esheshayo, isampula yokulawula i-positive ayikhiqizi ijeli?
Okokuqala, sebenzisa amanzi okuhlola e-endotoxin ebhaktheriya ukuze uqinisekise ama-lysate reagent, uqinisekise ukuthi opharetha nendawo ezungezile bayahlangabezana nemithethonqubo;

Uma ikhithi ifanelekile, khona-ke ukulawulwa okuhle kwesampula akukwenzi ukwakheka kwejeli ngenxa yomphumela wokuvimbela wesampula, futhi isampula kufanele icutshungulwe futhi iphathwe.
Indlela evame kakhulu yokucubungula isampula yi-dilution.