I-LAL Reagent noma i-TAL Reagent yokuhlolwa kwe-endotoxin

I-Limulus amebocyte lysate (LAL) noma i-Tachypleus tridentatus lysate (TAL) iwukukhipha okunamanzi kwamangqamuzana egazi asuka kunkalankala yesicathulo.

Futhi ama-endotoxins ama-molecule e-hydrophobic ayingxenye ye-lipopolysaccharide complex eyakha ingxenye enkulu ye-membrane yangaphandle ye-Gram-negative bacteria.Imikhiqizo yabazali engcoliswe ama-pyrogens ingaholela emiphumeleni emibi njengokushisa, ukushaqeka, ukwehluleka kwezitho, noma ngisho nokufa.

I-reagent ye-LAL/TAL ingasabela nge-endotoxin yebhaktheriya kanye ne-lipopolysaccharide (LPS).Ikhono lokubopha i-endotoxin ye-LAL kanye nokujiyisa yilo eliyenza ibaluleke kakhulu embonini yethu yezemithi.Futhi yingakho i-reagent ye-LAL/TAL ingase isetshenziswe ukuze kutholwe noma kulinganise i-endotoxin yebhaktheriya.

Ngaphambi kokuthola ukuthi i-LAL/TAL ingase isetshenziselwe ukuhlola i-endotoxins yebhaktheriya, onogwaja baqashwa ukuze bathole futhi balinganise ubuthi be-endotoxin emikhiqizweni yezemithi.Uma kuqhathaniswa ne-RPT, i-BET ene-LAL/TAL reagent iyashesha futhi iyasebenza, futhi iyindlela edumile yokwenza ukuqapha okunamandla kokugxilwa kwe-endotoxin embonini yezemithi, njalonjalo.

I-gel clot endotoxin test assay, eyaziwa nangokuthi i-Limulus Amebocyte Lysate (LAL) test, noma ebizwa nge-Lyophilized Amebocyte Lysate (LAL) iyindlela esetshenziswa kakhulu yokuthola kanye nokulinganisa ubuthi be-endotoxin emikhiqizweni eyahlukene, ikakhulukazi embonini yezemithi nemishini yezokwelapha.Kubhekwa njengesixazululo esidingekayo emkhakheni wokutholwa kwe-endotoxin ngenxa yokusebenza kwayo kahle nokwamukelwa kokulawula.

Ukuhlolwa kwe-LAL kusekelwe esimisweni sokuthi amangqamuzana egazi wezinkalankala zehhashi (i-Limulus polyphemus noma i-Tachypleus tridentatus) aqukethe isici sokujiyisa esisabela nge-endotoxin yebhaktheriya, okuholela ekwakhekeni kwehlule elifana nejeli.Lokhu kusabela kuzwela kakhulu futhi kuqonde ngqo kuma-endotoxins, okuyizingxenye ezinobuthi zolwelwesi lwangaphandle lwebhaktheriya e-gram-negative.

Kunezizathu ezimbalwa zokuthi kungani ukuhlolwa kwe-gel clot endotoxin kubhekwa njengesixazululo esidingekayo ekutholweni kwe-endotoxin:

1. Ukwamukelwa Okungokomthetho: Ukuhlolwa kwe-LAL kuyaqashelwa futhi kwamukelwe iziphathimandla ezilawulayo ezifana ne-United States Pharmacopeia (USP) kanye ne-European Pharmacopoeia (EP) njengendlela evamile yokuhlolwa kwe-endotoxin.Ukuhambisana nale mithethonqubo kuyisibopho sokuqinisekisa ukuphepha kanye nekhwalithi yemikhiqizo yemithi.

2. Ukuzwela kanye Nokucaciswa: Ukuhlolwa kwe-LAL kunokuzwela okuphezulu, okuvumela ukutholwa kwamazinga aphansi kakhulu e-endotoxin.Iyakwazi ukuthola ukugxila kwe-endotoxin ephansi njengeyunithi ye-endotoxin engu-0.01 ngemililitha ngayinye (EU/mL).Ukucaciswa kokuhlolwa kuqinisekisa ukuthi ngokuyinhloko kuthola ama-endotoxin futhi kunciphisa imiphumela enezimanga.

3. Ukusebenza Kwezindleko: Ukuhlolwa kokuhlolwa kwehlule lejeli endotoxin ngokuvamile kubhekwa njengesixazululo sezomnotho uma kuqhathaniswa nezinye izindlela ezifana nokuhlolwa kwe-chromogenic noma i-turbidimetric.Idinga ama-reagents ambalwa kanye nemishini, ukunciphisa izindleko zokuhlola zizonke.Ngokwengeziwe, ukutholakala kwama-reagents e-LAL ajwayelekile emakethe kwenza kube lula ukuthi amalabhorethri enze ukuhlolwa.

4. Izinga Lemboni: Ukuhlolwa kwe-LAL kwamukelwe kabanzi embonini yezemithi nemishini yezokwelapha njengendlela evamile yokutholwa kwe-endotoxin.Kuyingxenye ebalulekile yezinqubo zokulawula ikhwalithi ngesikhathi sokwenziwa kwemikhiqizo yezemithi namathuluzi ezokwelapha, okuqinisekisa ukuthotshelwa kwezidingo zokulawula.

Nokho, kubalulekile ukuqaphela ukuthi ukuhlolwa kokuhlolwa kwehlule lejeli endotoxin kungase kube nemikhawulo, njengokuphazanyiswa kwezinto ezithile kanye nethuba lemiphumela enamanga noma engemihle.Ezimweni ezithile, ezinye izindlela ezifana ne-chromogenic noma i-turbidimetric assays zingase zisetshenziselwe ukugcwalisa noma ukuqinisekisa imiphumela etholwe ekuhlolweni kwe-LAL.