Amanzi Angenayo I-Endotoxin: Ukudlala Indima Ebalulekile Ekuhlolweni Kwe-Endotoxin
Isingeniso:
Ukuhlolwa kwe-Endotoxin kuyingxenye ebalulekile yezimboni ezihlukahlukene, okuhlanganisa ezemithi, izisetshenziswa zezokwelapha, kanye ne-biotechnology.Ukutholwa okunembile nokuthembekile kwama-endotoxin kubalulekile ukuze kuqinisekiswe ukuphepha komkhiqizo nokuhambisana nezindinganiso zokulawula.Isidingo esisodwa esibalulekile sokuhlola i-endotoxin ukusetshenziswa kwamanzi angenayo i-endotoxin.Kulesi sihloko, sizohlola ukubaluleka kwamanzi angenayo i-endotoxin, indima yawo ekwenzeni i-Lyophilized Amebocyte Lysate (LAL) ukuhlolwa kwe-endotoxin, kanye nokubaluleka kokusebenzisa amanzi angenayo i-endotoxin ekuhlolweni kwe-Bacterial Endotoxin Test (BET).
Ukuqonda i-Endotoxins:
Ama-endotoxins ama-lipopolysaccharides (LPS) atholakala kulwelwesi lwangaphandle lwebhaktheriya e-Gram-negative.Bangabalamuli abanamandla bokuvuvukala futhi bangabangela imiphumela emibi kakhulu uma ikhona emikhiqizweni yemithi noma emishinini yezokwelapha.Ngenxa yamandla abo okubangela ukusabela kwe-pyrogenic, ukutholwa okunembile kanye nomthamo we-endotoxins kubalulekile.
Ukuhlolwa kwe-LAL Endotoxin:
Indlela eyaziwa kakhulu yokuhlola i-endotoxin i-LAL assay, esebenzisa igazi lenkalankala yehhashi.I-Limulus polyphemus ne-Tachypleus tridentatus.I-reagent ye-Lyophilized Amebocyte Lysate (LAL) ikhishwa emangqamuzaneni egazi alezi zinkalankala, aqukethe iphrotheni ejiyisayo esebenza lapho kukhona ama-endotoxins.
Iqhaza likaAmanzi Angenayo I-EndotoxinEkuhlolweni kwe-LAL:
Amanzi ayingxenye eyinhloko ekulungiseleleni i-reagent kanye nezinyathelo zokuhlanjululwa kokuhlolwa kwe-LAL.Kodwa-ke, ngisho namanani okulandelela ama-endotoxin akhona emanzini kampompi avamile angaphazamisa ukunemba nokuzwela kokuhlolwa.Ukuze unqobe le nselele, amanzi angenayo i-endotoxin kufanele asetshenziswe kuyo yonke inqubo yokuhlola.
Amanzi angenayo i-Endotoxin adlala indima ebalulekile ekuqinisekiseni ukuthi izithasiselo ezisetshenziswa ekuhlolweni kwe-LAL azingcoliswanga yi-endotoxin.Ngaphezu kwalokho, ivimbela imiphumela engemihle noma engemihle engamanga, ngaleyo ndlela inikeze ukulinganisa okuthembekile nokunembayo kwe-endotoxin.
Ukukhetha Amanzi Alungile Okuhlolwa Kwe-LAL:
Ukuthola amanzi angenayo i-endotoxin, izindlela ezimbalwa zokuhlanza zingase zisetshenziswe.I-deionization, i-distillation, kanye ne-osmosis ehlanekezelwe yizindlela ezivame ukusetshenziswa zokunciphisa ubukhona be-endotoxins emanzini.Lezi zindlela zisusa ukungcola okuhlukahlukene, okuhlanganisa nama-endotoxins atholakala kubhaktheriya.
Ukwengeza, kubalulekile ukuqinisekisa ukuthi iziqukathi ezisetshenziselwa ukugcina, ukuqoqa, nokusabalalisa amanzi angenayo i-endotoxin ziqinisekiswa ngokufanelekile futhi azingcoliswa yi-endotoxin.Lokhu kuhlanganisa ukusebenzisa amashubhu, amabhodlela, nezihlungi ezingenayo i-endotoxin phakathi nenqubo.
Ukubaluleka Kwamanzi E-BET:
KweUkuhlolwa kwe-bacterial Endotoxin (BET), amanzi angenayo i-endotoxin, aziwa nangokuthi amanzi e-BET, asetshenziswa njengesilawuli esingalungile ukuze kuqinisekiswe ukuzwela nokucaciswa kokuhlolwa kwe-LAL.Amanzi e-BET kufanele aqukathe izinga elingabonakali lama-endotoxin, aqinisekise ukuthi noma yimuphi umsebenzi we-endotoxin olinganisekayo utholakala kuphela kusampula elihloliwe.
Ukusetshenziswa kwamanzi e-BET ekuhlolweni kwe-endotoxin kusebenza njengokulawula okubalulekile ukuze kuqinisekiswe ukusebenza kahle kwama-reagents e-LAL, uhlelo lokuhlola, kanye nemishini.Lesi sinyathelo sokuqinisekisa sibalulekile ukuze kuhlolwe ngokunembile ubukhona nokugxila kwama-endotoxin kusampula elihloliwe.
Isiphetho:
Amanzi angenayo i-endotoxin adlala indima ebalulekile ekutholakaleni okunembile nokuthembekile kwe-endotoxins ezimbonini ezihlukahlukene.Ekuhlolweni kwe-LAL endotoxin, iqinisekisa ukuthi izithasiselo ezisetshenziswayo azingcolisiwe, zinikeza ukulinganisa okunembile.Ku-BET, amanzi angenayo i-endotoxin asebenza njengokulawula, aqinisekisa ukuzwela kokuhlolwa kwe-LAL.Ngokunamathela ezindleleni zokuhlanza eziqinile nokusebenzisa iziqukathi eziqinisekisiwe, amandla emiphumela namaphutha angamanga angancishiswa kakhulu.
Njengoba ukubaluleka kokuhlolwa kwe-endotoxin kuqhubeka nokukhula, indima yamanzi angenayo i-endotoxin iba semqoka nakakhulu.Ukusebenzisa amasu athembekile okuhlanza amanzi kanye nokuhlanganisa izinqubo ezihamba phambili ohlelweni lokuhlola kuzoqinisekisa ukuphepha nokuhambisana kwemikhiqizo yezemithi, izinto zezokwelapha, nezinye izinto ezizwela i-endotoxin.
Isikhathi sokuthumela: Nov-29-2023
