Ukuhlolwa kwe-Recombinant Factor C (rFC).iyindlela esetshenziswa kakhulu yokuthola ama-endotoxins ebhaktheriya, eyaziwa nangokuthi i-lipopolysaccharides (LPS), i-Endotoxins iyingxenye yolwelwesi lwangaphandle lwamabhaktheriya e-Gram-negative angabangela ukusabela okunamandla kokuvuvukala ezilwaneni, kuhlanganise nabantu.Ukuhlolwa kwe-rFC kusekelwe ekusetshenzisweni kohlobo olusungulwe ngokofuzo lwe-Factor C, i-enzyme etholakala ngokwemvelo egazini lenkalankala yehhashi futhi ebandakanyeka endleleni yokujiyisa.Ekuhlolweni kwe-rFC, i-Recombinant Factor C isetshenziselwa ukuthola ukuba khona kwama-endotoxins ngokulinganisa Ngokukala okuqukethwe kwama-substrates aqhekeziwe lapho kukhona i-endotoxin.Uma kuqhathaniswa nezindlela zendabuko zokutholwa kwe-endotoxin, njenge-Limulus Amebocyte Lysate (LAL) assay esebenzisa igazi lenkalankala yehhashi, ukuhlolwa kwe-rFC kubhekwa njengokujwayelekile kakhulu futhi kukhiqizeka kabusha, njengoba kungancikile ekusetshenzisweni kwezithako eziphuma ezilwaneni.Ukuhlolwa kwe-rFC nakho kuvumelana nemvelo futhi kuzinzile, njengoba kunciphisa isidingo sokuqoqwa nokusetshenziswa kwezinkalankala zezicathulo zamahhashi ekutholeni i-endotoxin.
Ukuhlolwa kwe-rFC kuvunywe iziphathimandla ezilawulayo, njenge-United States Pharmacopeia (USP) , i-European Pharmacopoeia (EP) kanye ne-Chinese Pharmacopoeia (CP) ukuze zisetshenziswe ekuhlolweni kokulawulwa kwekhwalithi kwemithi nemishini yezokwelapha.
Izinzuzo ze-recombinant factor c assay
Ukuhlolwa kwe-Recombinant Factor C (rFC) kunikeza izinzuzo ezimbalwa kunezindlela zendabuko zokuthola ama-endotoxins, njenge-Limulus Amebocyte Lysate (LAL) assay.Ezinye zezinzuzo ze-rFC assay zifaka:
1. Ukulinganisa: I-rFC assay ubuchwepheshe be-DNA obuphinda busetshenziswe obusebenzisa iphrotheni eyodwa, echazwe njengesisetshenziswa esitholwayo.Lokhu kwenza i-assay ifane kakhulu futhi ingajwayele ukuhlukahluka uma iqhathaniswa ne-LAL assay, encike ekusetshenzisweni kwengxube eyinkimbinkimbi yamaphrotheni akhishwe egazini lenkalankala yehhashi.
2. Ukukhiqiza kabusha: Ukuhlolwa kwe-rFC kunamazinga aphezulu okuphindaphindeka, njengoba kusebenzisa iphrotheni eyodwa, echazwe njengesisebenzi esitholwayo.Lokhu kuvumela imiphumela engaguquki, ngisho nakumaqoqo ahlukene nama-reagents amaningi.
3. Ukusetshenziswa kwezilwane okuncishisiwe: Ukuhlolwa kwe-rFC kuyindlela enobungani nemvelo futhi esimeme yokuthola i-endotoxin, njengoba ingadingi ukusetshenziswa kwezilwane eziphilayo noma ezihlatshiwe, njengezinkalankala zehhashi.
4. Imali engabizi kakhulu: Ukuhlolwa kwe-rFC kuvame ukonga kakhulu kune-LAL assay, ngenxa yesidingo esincishisiwe sezilwane eziphilayo kanye nesimo esimisiwe sokuhlola.
5. Ukuzinza: Ukuhlolwa kwe-rFC kuqinile futhi kungasetshenziswa ezinhlelweni eziningi ezahlukahlukene, okuhlanganisa ukuhlolwa kokulawulwa kwekhwalithi kwemithi, izisetshenziswa zezokwelapha, neminye imikhiqizo engase ibe ne-endotoxin.
6. Ukugunyazwa ngokomthetho: Ukuhlolwa kwe-rFC kuvunywe iziphathimandla ezilawulayo ezifana ne-United States Pharmacopeia (USP) , i-European Pharmacopoeia (EP) kanye ne-Chinese Pharmacopoeia (CP) ukuze zisetshenziswe ekuhlolweni kokulawulwa kwekhwalithi kwemithi nemishini yezokwelapha.Lokhu kunikeza izinga eliphezulu lokuzethemba ekuthembekeni nasekunembeni kokuhlolwa.
Ukuze kuhlangatshezwane nezidingo ezihlukahlukene, i-Bioendo iphinde ikhiqize futhi inikeze indlela yendabuko yekhithi yokuhlola ihlule lejeli endotoxin, ikhithi yokuhlola ihlule elisheshayo, ikhithi yokuhlola i-endotoxin yobuningi kuhlanganise “kinetic turbidimetric endotoxin test assay kitfuthiikhinetic chromogenic endotoxin test assay kit” .
Isikhathi sokuthumela: Feb-19-2023
