Iyini i-Endotoxin

Ama-Endotoxins ama-molecule amancane atholakala kubhaktheriya e-hydrophobic lipopolysaccharides (LPS) atholakala kulwelwesi lwamaseli olungaphandle lwebhaktheriya e-gram-negative.Ama-Endotoxins aqukethe iketango le-polysaccharide eliwumongo, amaketanga aseceleni e-O-specific polysaccharide (O-antigen) kanye nengxenye ye-lipid, i-Lipid A, enesibopho semiphumela yobuthi.Amagciwane achitha i-endotoxin ngenani elikhulu lapho amangqamuzana efa nalapho ekhula futhi ehlukana.I-Escherichia coli eyodwa iqukethe cishe ama-molecule e-LPS ayizigidi ezimbili iseli ngalinye.

I-Endotoxin ingangcolisa kalula ama-labware, futhi ubukhona bayo bungadlulisela ngokuphawulekayo kokubili ukuhlolwa kwe-in vitro kanye ne-vivo.Futhi emikhiqizweni yabazali, imikhiqizo yabazali engcoliswe ama-endotoxins okuhlanganisa i-LPS ingaholela ekuthuthukisweni kwemfiva, ukungeniswa kokusabela kokuvuvukala, ukushaqeka, ukwehluleka kwezitho kanye nokufa kumuntu.Ngemikhiqizo ye-dialysis, i-LPS ingadluliselwa nge-membrane enosayizi omkhulu we-pore ngokuhlunga emuva kusuka ku-dialysis fluid kuya egazini, izinkinga zokuvuvukala zingabangelwa ngokufanele.

I-Endotoxin itholwa yi-Lyophilized Amebocyte Lysate (TAL).I-Bioendo inikezelwe ekucwaningeni, ekuthuthukiseni nasekukhiqizeni i-reagent ye-TAL iminyaka engaphezu kwamashumi amane.Imikhiqizo yethu ihlanganisa wonke amasu asetshenziswa ukuthola i-endotoxin, okuyindlela ye-gel-clot, indlela ye-turbidimetric, kanye nendlela ye-chromogenic.