Kuyini ukuhlolwa kwe-Endotoxins?
Ama-endotoxins ama-molecule e-hydrophobic ayingxenye ye-lipopolysaccharide complex akha ingxenye enkulu yolwelwesi lwangaphandle lwebhaktheriya e-Gram-negative.Akhululwa lapho amagciwane efa futhi ulwelwesi lwawo lwangaphandle luhlakazeka.Ama-endotoxins athathwa njengabanegalelo elikhulu ekuphenduleni kwe-pyrogenic.Futhi imikhiqizo yabazali engcoliswe ama-pyrogens ingaholela ekuthuthukiseni umkhuhlane, ukufakwa kwempendulo yokuvuvukala, ukushaqeka, ukwehluleka kwezitho nokufa kubantu.
Ukuhlolwa kwe-Endotoxins ukuhlolwa kokuthola noma ukulinganisa ama-endotoxins avela kubhaktheriya e-Gram-negative.
Onogwaja baqashwe ukuze bathole futhi balinganise i-endotoxins emikhiqizweni yemithi ekuqaleni.Ngokusho kwe-USP, i-RPT ibandakanya ukuqapha ukwenyuka kwezinga lokushisa noma imfiva ngemva kokujova ngomjovo wemithi konogwaja.Futhi i-21 CFR 610.13(b) idinga ukuhlolwa kwe-pyrogen kanogwaja emikhiqizweni ethile yebhayoloji.
Ngawo-1960, uFredrick Bang noJack Levin bathola ukuthi ama-amebocyte enkalankala yehhashi azojiya lapho kukhona ama-endotoxins.II-Limulus Amebocyte Lysate(noma i-Tachypleus Amebocyte Lysate) yathuthukiswa ngokufanele ukuze ithathe indawo ye-RPT eminingi.Ku-USP, ukuhlolwa kwe-LAL kubizwa ngokuthi ukuhlolwa kwe-bacterial endotoxin (BET).Futhi i-BET ingenziwa ngokusebenzisa amasu ama-3: 1) indlela yehlule lejeli;2) inqubo ye-turbidimetric;3) inqubo ye-chromogenic.Izidingo zokuhlolwa kwe-LAL ziqukethe i-pH elungile, amandla e-ionic, izinga lokushisa, nesikhathi sokufukamela.
Uma kuqhathaniswa ne-RPT, i-BET iyashesha futhi isebenza kahle.Nokho, i-BET ayikwazanga ukumiselela i-RPT ngokuphelele.Ngoba ukuhlolwa kwe-LAL kungase kuphazanyiswe izici futhi akukwazi ukubona ama-non-endotoxin pyrogens.
Isikhathi sokuthumela: Dec-29-2018
