Isixazululo Sokuhlola I-Endotoxin Elinganiselwe Ye-WFI noma i-Dialysate
I-Dialysis (Indlela encane ye-Kinetic Chromogenic)
Ngawo-1970, isigameko esibi se-hemodialysis "se-pyrogenic reaction" saqubuka e-United States.Uphenyo lwakamuva lwathola ukuthi i-dialysate yayingcoliswe amagciwane e-Gram-negative.Ukusabela kwe-pyrogen okubangelwa i-hemodialysis ngokuyinhloko ukuthi i-pyrogen ku-dialyzer kanye namanzi e-dialysis idlula izinga, futhi okuqukethwe kwe-pyrogen egazini lesiguli kuyakhuphuka phakathi nenqubo yokucubungula, okubangela uchungechunge lwezimpawu.Ukuze kugwenywe ukusabela okungekuhle kwekhwalithi yamanzi ezigulini, ukumiswa kwe-dialysate, ukugezwa kwe-dialysate, namanzi asetshenziselwa i-dialyzer namapayipi egazi konke kudinga ukusetshenziswa kwamanzi angenalutho akhishwe yi-dialysis, okungukuthi amanzi e-dialysis.Izinga lenhlanzeko lamanzi e-dialysis lihlobene nempilo kanye nezinga lempilo yeziguli eziningi ezinokwehluleka kwezinso.Ngakho-ke, kubalulekile ukulawula izinga lamanzi ahlobene ne-dialysis.
I-Lysate reagent micro KC esebenzisa i-kinetic chromogenic micro-technology idinga kuphela u-25 μl wesampula no-25 μl we-Lysate reagent ekuhlolweni ngakunye.Inekhono eliqinile lokulwa nokuphazamiseka kanye nokuzwela kufika ku-0.005EU/ml.Ilungele kakhulu ukutholwa kwe-endotoxin yemikhiqizo ehlobene ne-dialysis.Ifakwe ngezifiso i-micro-detection 96-well plate/slat kanye nohlelo lokubona ngokushesha lwe-lysate microorganism ELx808, olubona ukutholwa kwe-endotoxin ye-micro-quantification ye-chromogenic, futhi isistimu ithola ngokuzenzakalelayo futhi ihlaziye esinyathelweni esisodwa.
Imikhiqizo ehlobene ekusebenzeni kwe-micro kinetic chromogenic endotoxin test assay:
Ikhithi ye-MKC: MKC0505VS;I-MKC0505V;I-MKC0505AS;I-MKC0505A, MPMC96.
Ibhodlela lesampula elingenayo i-Endotoxin, inombolo yekhathalogi PA10, ivolumu engu-10ml, isisombululo sevolumu enkulu sizonikezwa.
Amashubhu okuhlola angenayo i-Endotoxin, inombolo yekhathalogi T107540 kanye ne-T1310018
Ama-microplates angenayo i-Endotoxin (ekhiphekayo/engakhipheki), inombolo yekhathalogi MPMC96, imicu engu-8.
Amathiphu angenayo i-Endotoxin (1000ul kanye ne-250ul), inombolo yekhathalogi PT25096 noma PT100096
Lawula I-Endotoxin Ejwayelekile, inombolo yekhathalogi CSE10V
BET amanzi, Ikhathalogi inombolo TRW50 noma TRW100
Isifundi se-Microplate: ELx808
Sinikeza ikhithi yokuhlola i-Bioendo KT endotoxin, i-ELx808, i-vortex mixer kanye nemicu ye-microplate engena-pyrogen yesixazululo sokulinganisa i-endotoxin ye-WFI noma i-Dialysate.