I-Recombinant Cascade Reagents Isithombe Esifakiwe Se-Chromogenic Assay

I-Recombinant Cascade Reagents I-Chromogenic Assay

Ikhithi Yokuhlola I-Bioendo rCR Endotoxin (I-Recombinant Cascade Reagents I-Chromogenic Assay) isebenzisa ubuchwepheshe be-gene recombinant ukuveza indlela ye-Factor C i-serine zymogen proteases ye-horseshoe crab amebocyte kumaseli e-eukaryotic.Ama-reagents e-cascade afaka i-Factor C engavulwa yi-bacterial endotoxins, i-Factor B kanye ne-proclotting enzyme.Ama-Endotoxins abopha futhi aguqule i-Factor C efomini layo elisebenzayo, okuthi kamuva isebenze i-Factor B, yona eyenza isebenze i-enzayimi evalayo.I-enzayimu evimbezelayo ecushiwe bese ihlukanisa i-chromogenic substrate, ikhiphe i-pNA enombala ophuzi.I-pNA ekhishiwe ingalinganiswa nge-photometrically ku-405 nm.Ngokusekelwe kulokho, ukugcwala kwe-endotoxin kwesampula yokuhlola kungalinganiswa.Ikhithi yokuhlola i-rCR endotoxin ayisebenzisi izinto eziwumthombo wezilwane, ivikela izinkalankala zehhashi.

Uphenyo

Imininingwane Yomkhiqizo

Izici:

1.Ukutholwa okulula: ithuluzi lokubona elifanayo njenge-reagent ye-Chromogenic LAL isetshenziswa, futhi asikho isidingo sokumiselela ithuluzi lokuthola.2.Ukuzwela okuphezulu: ukuzwela kungaba phezulu njenge-0.001EU/ml.

I-3.Ukuzinza: ukuphindaphinda okuhle phakathi kwamaqoqo okukhiqiza

4.Ukucaciswa: ayisabeli nge-fungal (1,3)-β-D-glucan, igwema ukuhlehliswa okungamanga okubangelwa yi-factor G bypass.

Oda ulwazi:

Inombolo yekhathalogi

Ukucaciswa

Ibanga lokutholwa

I-RCR0428S

4 Recombinant Cascade Reagents — 2.8ml/vial

I-4 Buffer Buffer — 3.0ml/vial

2 Lawula I-Endotoxin Ejwayelekile — CSE10V

2 Amanzi e-BET — 50ml/vial

0.001-10EU/ml

RCR0428

0.005-5EU/ml

Okwedlule: Ikhithi Yokuhlola I-Bioendo™ rFC Endotoxin (I-Recombinant Factor C Fluorometric Assay)

Olandelayo:

Shiya Imilayezo Yakho

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