Ikhithi Yokuhlola I-Bioendo™ rFC Endotoxin (I-Recombinant Factor C Fluorometric Assay)

I-Bioendo™ rFC Endotoxin Test Kit(I-Recombinant Factor C Fluorometric Assay) isebenzisa indlela yokuhlanganiswa kabusha kofuzo ukuveza iphrotheni ye-serine protease catalytic cascade i-Factor C ehlangana ne-endotoxinI-TachypleusAmebocyte kumaseli eukaryotic.Ngemva kokubophezela nge-endotoxin, i-Factor C ephinde yahlanganiswa iyaguqulwa isuke ku-proteasogen engasebenzi iye kwi-bioactive protease, ebona futhi ibambe i-substrate ye-Fluorometric engezansi ukuze ikhiqize amasignali e-Fluorometric.Ukuqina kwesiginali ye-fluorescence kuhlotshaniswa kahle nokugcwala kwe-endotoxin, ngakho-ke i-endotoxin ingatholwa ngobuningi.Ukuhlolwa kabusha kwe-Factor C endotoxin akusebenzisi i-amebocyte ye-horseshoe crab amebocyte, evikela izinsiza ze-horseshoe crab.Kuyisizukulwane esisha sobuchwepheshe bokuhlola i-endotoxin.

Uphenyo

Imininingwane Yomkhiqizo

I-Bioendo™Ikhithi Yokuhlola ye-rFC Endotoxin(I-Recombinant Factor C Fluorometric Assay)

Ithuluzi elidingekayo: Isifundi se-microplate esisebenza ngobuchwepheshe be-incubating fluorescence siyadingeka esinobude beza elivusa amadlingozi 380nm/izihlungi ezikhishwayo ze-wavelength 440nm (sicela uxhumane nomnyango wethu wokuthengisa ukuze uthole imininingwane).

Izici:

1. Ukusabela Okusheshayo: Imizuzu engama-30 - 60 ukuqedela ukutholwa

2. Ukucaciswa kwe-Endotoxin: isabela kuphela nge-endotoxin, igwema ukuphazamiseka kwe-beta-glucan

3. Ukuzwela okuphezulu: ukutholwa okuncane kungafinyelela ku-0.005EU/ml

4. Ukuphindaphinda kwenqwaba: inkulumo ehambisanayo, ukuphindaphinda okuhle phakathi kwenkatho

5. Ama-reagents asesimweni se-lyophilized, kulula ukuwagcina nokuthutha

6. Kulula ukuyisebenzisa, isistimu ithola ngokuzenzakalelayo futhi ihlaziye ngesinyathelo esisodwa.

Thenga isincomo

Inombolo yekhathalogi	Ukucaciswa	Ibanga lokutholwa
I-RFC96TS	I-2 Recombinant Factor C -- 2.5ml/vial;I-2 Buffer Buffer -- 3.0ml/ibhodlela; 2 Lawula I-Endotoxin Ejwayelekile -- CSE10V; Amanzi angu-1 e-BET -- TRW50, 50ml; 15 emnyama engena-endotoxin; 8-umthombo microplate strip; 1 imivimbo bracket; 96Isivivinyo/Ikhithi.	0.005-5EU/ml
RFC96T		0.01-10EU/ml
I-MPF96	I-Endotoxin engenawo i-Endotoxin yepuleti elimnyama elinemithombo engu-8 (ye-Recombinant Factor C Fluorometric Assay)

Okwedlule: (1-3)-β-D-Glucan Detection Kit (Indlela ye-Kinetic Chromogenic)

Olandelayo: I-Recombinant Cascade Reagents I-Chromogenic Assay

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