I-Gel Clot Lyophilized Amebocyte Lysate Multi-test Vial G17

I-Gel Clot Lyophilized Amebocyte Lysate (LAL) Multi-test Vial, uchungechunge lwe-G17

1. Ulwazi Lomkhiqizo

I-Gel Clot I-Amebocyte Lysate Multi-test Vial iyi-reagent ye-Lyophilized Amebocyte Lysate ekhetha indlela yehlule lejeli ukuthola i-endotoxin noma i-pyrogen.Njengendlela esabalele, ukuhlolwa kwe-gel-clot kwe-endotoxin kulula futhi akudingi ithuluzi elithile nelibizayo.I-Bioendo inikeza i-Gel Clotukuhlolwa kwe-endotoxinikhithi ku-1.7ml ibhodlela ngayinye.

2. Ipharamitha yomkhiqizo

Ibanga lokuzwela: 0.03EU/ml, 0.06EU/ml, 0.125EU/ml, 0.25EU/ml, 0.5 EU/ml

3. Isicelo soMkhiqizo

Ukufaneleka komkhiqizo we-endotoxin (pyrogen), amanzi omjovoukuhlolwa kwe-endotoxin, impahla eluhlazaukuhlolwa kwe-endotoxinnoma ukuqapha izinga le-endotoxin phakathi nenqubo yokukhiqiza yezinkampani ezenza imithi noma abakhiqizi bemishini yezokwelapha.

Qaphela: I-Lyophilized Amebocyte Lysate (i-LAL reagent) ekhiqizwa yi-Bioendo yenziwe nge-lysate ye-amebocytes (amangqamuzana egazi amhlophe) kusuka kunkalankala yehhashi.

Indlela ye-gel clotIsivivinyo se-LAL, i-reagent ye-lysate ehlanganisiwe ithola okungenani izivivinyo eziyi-16 ngevial ​​ngayinye:

Isimo somkhiqizo:

Ukuzwela kwe-Lyophilized Amebocyte Lysate reagent kanye namandla e-Control Standard Endotoxin ayahlolwa ngokumelene ne-USP Reference Standard Endotoxin.Ikhithi ye-Lyophilized Amebocyte reagent iza nemiyalelo yomkhiqizo, Isitifiketi Sokuhlaziya.

Esinye sezici ezibalulekile zochungechunge lwe-Bioendo Lysate reagent G17 ukumelana kwayo okuqinile nokuphazamiseka.Lokhu kubaluleke kakhulu uma usebenza ngamasampula amakhulu osayizi, njengoba kunethuba eliphezulu lokuphazamiseka okuvela kwezinye izinto ezikhona kusampula.Iukutholwa kwe-endotoxininqubo idinga izinga eliphezulu lokuzwela kanye nokucaciswa, futhi i-reagent ye-Bioendo Lysate yenziwe ngokukhethekile ukuze kuncishiswe umthelela wezinto eziphazamisayo.Lokhu kuqinisekisa ukuthi inqubo yokuthola i-endotoxin inembile futhi inokwethenjelwa, ngisho nalapho isebenza ngamasampula amakhulu osayizi.

Ngaphezu kokumelana nokuphazamiseka, i-Gel Clot Method Lyophilized Amebocyte Lysate Multi-test Vial G17 nayo yakhelwe ukuthi isetshenziswe kalula.Ifomethi ye-lyophilized ye-reagent ivumela ukuzinza kwesikhathi eside nokugcinwa okulula, kuyilapho ifomethi ye-vial yokuhlola eminingi ivumela ukuhlolwa okulungile kwamasampuli amaningi.Lokhu kwenza i-reagent ye-Bioendo Lysate ibe ukukhetha okusebenzisekayo nokungabizi kakhulu kokutholwa kwe-endotoxin ezinhlelweni ezinkulu, njengokukhiqizwa kwemithi nokukhiqizwa kwemishini yezokwelapha.Sekukonke, iUkuhlolwa kwe-Gel Clot LAL, usebenzisa i-reagent ye-Bioendo Lysate, iyithuluzi elibalulekile lokuqinisekisa ukuphepha kanye nekhwalithi yemikhiqizo ngokuthola ukungcoliswa kwe-endotoxin ngamanani amakhulu.

Kungani izazi ze-microbiologists eziningi zikhetha uchungechunge lwekhithi ye-gel clot assay G17?

1. Uchungechunge lwe-LAL reagent G17 luyindlela ejwayelekile kunazo zonke yokubonisa ubukhona be-endotoxin ngokusekelwe ekutheni ukwakheka kwejeli kushubhu yesampula uma kusetshenziswaukuhlolwa kwe-bacterial endotoxin.

2. Eziningiukuhlolwa kwe-limulus lysateukuze kutholwe ubuthi be-endotoxin emithini yomzali, lapho kutholwa amasampula yenani elilingene.

3. Inqubo yokuhlola ye-LAL yokufukamela, idivayisi elula ukugeza ngamanzi noma i-incubator yokushisa eyomile.

4. Ikhwalithi ephezulu yokugcina ye-endotoxin free tube (<0.005EU/ml) kanye nekhwalithi ephezulu ithiphu elingenayo i-pyrogen (<0.005EU/ml) njengento esetshenziswayo eqinisekisiwe ukuze kuqinisekiswe umphumela olungile.

Imikhiqizo ehlobene ekuhlolweni kokuhlolwa kwe-endotoxin:

• Amanzi Okuhlola I-Bacterial Endotoxins Test (BET), Ncoma i-TRW50 noma i-TRW100
• Ishubhu yengilazi yamahhala ye-Endotoxin (i-dilution tube), Ncoma i-T1310018 ne-T107540
• Amathiphu wamahhala we-Pyrogen, Ncoma i-PT25096 noma i-PT100096
• Pipettor, Ncoma i-PSB0220
• I-Test Tube Rack
• Ithuluzi lokufukamela (Ukugeza Amanzi noma I-Incubator Yokushisa Eyomile ), Ncoma i-Dry Heat Incubator TAL-M2
• I-Vortex Mixter, Ncoma i-VXH.
• Lawula I-Endotoxin Ejwayelekile, i-CSE10V.

I-BioendoUkuhlolwa kwe-LAL kwama-pyrogens or Ukuhlolwa kwe-LAL endotoxin, futhiUkuhlolwa kwe-TAL, zonke izincazelo zingezesivivinyo se-endotoxin.

Ngokusekelwe kubuciko bokucubungula i-Bioendo, amakhithi ayi-lyophilized amebocyte lysate (LAL).

I-Limulus amebocyte lysate (I-LAL) i-aqueous extract yamangqamuzana egazi (ama-amoebocyte) ukusukaI-Atlantic horseshoe crab I-Limulus polyphemus.I-LAL ihlangana nebhaktheriyaendotoxin lipopolysaccharide(LPS), okuyi-aulwelwesiingxenye yeamagciwane e-gram-negative.Lokhu kusabela kuyisisekelo se-Ukuhlolwa kwe-LAL, esetshenziswa kabanzi ukuthola nokulinganisa amagciwaneama-endotoxins.

E-Asia, okufanayoI-Tachypleus amebocyte lysate (I-TAL) ukuhlolwa okusekelwe kuzinkalankala zendawo zamahhashiI-Tachypleus gigas or I-Tachypleus tridentatusisetshenziswa ngezikhathi ezithile kunalokho.Ii-recombinant factor C(rFC) ukuhlolwa yi-aesikhundleniye-LAL/TAL esekelwe ekuphenduleni okufanayo.