I-EtEraser™ HP Endotoxin Removal Kit

I-EtEraser™ SE Endotoxin Removal Kit

1. Ulwazi lomkhiqizo

Singochwepheshe bokuhlolwa kwe-endotoxin kanye ne-endotoxinremoval.Sinikeza izixazululo eziphelele ezihlanganisa lonke uhla lwe-endotoxincontrol, ukuqapha i-endotoxin nokuhlolwa.Imikhiqizo yethu ihlanganisa ukusetshenziswa kocwaningo lwaselabhorethriikhithi yokususa i-endotoxins futhi ingakhuliswa ifike kumakholomu esikali sokukhiqiza.I-Lipopolysaccharide (LPS) iyi-endotoxin yebhaktheriya futhi iyingxenye enkulu yezindonga zamangqamuzana amagciwane egram-negative.I-recombinantprotein evela ku-E.coli ngokuvamile iqukethe izinga eliphezulu lama-endotoxins.Ukususwa kwalawa ma-endotoxins kuyadingeka kakhulu ezinqubweni ezingezansi zomfula.

I-EtEraser HP Endotoxin Removal Kitzenzelwe ukususa ukungcoliswa kwe-endotoxin ezixazululweni ezinamanzi.Amakhithi aqukethe ama-resinbinds okukhipha i-endotoxin ukunciphisa amazinga e-endotoxin kumasampula amaprotheni ngo-≥99% ngaphansi kwamahora angu-2.

Le khithi yokususa i-endotoxin ingasetshenziswa kumaprotheni, i-DNA/RNA, i-polysaccharide namanye amasampula ebhayoloji.

2.Izici zomkhiqizo

◆ ukuzinza okuphezulu - akuphazamisi umsebenzi wesampula yezinto eziphilayo

◆ ukutholwa kwamaprotheni aphezulu — >95%ukuthola amaprotheni kumasampula amaprotheni

◆ ukusebenza kahle kokususa okuphezulu — susa>99% endotoxin, izinga le-endotoxin kusampula lingaba ngaphansi kwenqubo yokukhipha i-endotoxin engu-0.1 EU/mlafter

◆ uhla olubanzi lokusetshenziswa - lungasetshenziswa ekukhipheni i-endotoxin kumaprotheni, ama-peptide, amasosha omzimba, imijovo, i-polysaccharide namanye amasampula ebhayoloji.

Ikhithi yokususa i-endotoxin ye-EtEraserHP isebenzisa immobilized affinity ligandmodified PMB ukuze ibophe futhi isuse i-endotoxin esixukwini esinamanzi.I-modifiedPMB (i-polymyxin B) i-ligand iyi-ligand ethile ephezulu ebopha i-endotoxin.Ikholomu inezinga eliphezulu kakhulu lokutholwa kwamaprotheni ngaphezu kwama-95%.Ileveli ye-endotoxin kusampula ingaba ngaphansi kuka-0.1 EU/ml ngemva kwenqubo yokukhipha i-endotoxin.

I-kitin ihlanganisa ikholomu yokususa i-endotoxin epakishwe ngaphambili engu-1.5 ml, Isithambisi Sokulinganisa, Isithameli Sokuvuselela kanye neshubhu lokuqoqa elingenayo i-pyrogen namathiphu.Ikholomu inamandla okubopha aphezulu okuthi > 2, 000, 000 EU / ml.Lo mkhiqizo ungase usetshenziswe kabusha izikhathi ezinhlanu uma ukhiqizwa kabusha ngendlela efanele.I-affinity resin iyatholakala ngokungenasici futhi ingakhuphuka ngenqubo ye-biopharmaceutical.