Isiqondisookwenzelwe i-Bioendo End-point Chromogenic LAL Test Assay Kits:
I-TAL reagent, okungukuthi i-lyophilized amebocyte lysate ekhishwe egazini eliluhlaza lenkalankala yehhashi (i-Limulus polyphemus noma i-Tachypleus tridentatus), isetshenziswa njalo ukwenza ukuhlolwa kwe-bacterial endotoxins.
Kwa-Bioendo, sakha amakhithi okwenza i-assay ye-TAL yephoyinti lokugcina ye-chromogenic TAL, ehlanganisa zonke izinto ezisebenza kabusha ezidingekayo ku-BET ngoshintsho ekuthuthukisweni kombala, futhi sivumele ikhasimende ukuthi lithole imiphumela enembile ngesikhathi esifushane.
Izimiso zokutholwa kwe-chromogenic yephoyinti lokugcina nge-reagent ye-TAL yilezi: ukusabela kwe-enzymatic kwenzeka lapho i-endotoxin yebhaktheriya yenza i-factor C, futhi i-coagulase yakheka ngokufanele.Bese i-coagulase ihlukanisa i-substrate engenambala ukuze ikhiphe umkhiqizo onombala ophuzi i-pNA.I-pNA ekhishiwe ingalinganiswa nge-photometrically ku-405nm.Njengoba ukumunca kuhlotshaniswa kahle nokugxilwa kwe-endotoxin, ukugxila kwe-endotoxin kungase kulinganiswe ngokufanele.
I-EC64405, okungukuthi i-BioendoEC Endotoxin Test Kit (I-End-point Chromogenic Assay), iyiphoyinti lokugcina le-Chromogenic TAL assay / LAL kit engasetshenziswa ukuze kutholwe inani le-endotoxin yebhaktheriya ekhona kusampula.Ikhithi ye-EC64405 ekhiqizwe yi-Bioendo ivumela ukuhlolwa okungu-64, futhi izinga lokuzwela lisuka ku-0.1 EU/ml liye ku-1 EU/ml.I-Bioendo EC64405 iqukethe wonke ama-reagents adingekayo ukuze kuhlolwe i-chromogenic iphuzu lokugcina.Njengoba futhi sinezinsiza ezingenayo i-endotoxin noma ezingenayo i-pyrogen, singaphinda sipakishe ikhithi ngokusekelwe kulokho okudingayo.
I-Incubating Microplate Reader iyadingeka ukuze kuhlolwe i-chromogenic iphuzu lokugcina.Isifundi sethu se-microplate esifukamelayo i-ELx808IULALXH (imodeli entsha esacutshungulwa futhi izokwethulwa maduze) ivumela amasampula ahlukene ukuthi atholwe ku-microplate yemithombo engu-96 ngesikhathi esifanayo futhi izohlaziya ukutholwa kwe-endotoxin ngokuzenzakalelayo nangokunembile.
Iphoyinti lokugcina lokuhlola i-LAL ye-chromogenic enochungechunge olubili lwekhithi, eyodwa isebenzisa isifundi se-microplate ukufunda umphumela wokulinganisa nge-microplate engena-pyrogen, enye isebenzisa i-spectrophotometer evamile ukufunda umphumela wokulinganisa ngamashubhu engilazi angenayo i-endotoxin.
I-Lyophilized Amebocyte Lysate / LAL Reagent ekhiqizwa yi-Bioendo yenziwe ngegazi le-amebocyte lysate elitholakala kunkalankala yehhashi.
| Ikhathalogi No. | Incazelo | Okuqukethwe Kwekhithi | Ukuzwela (EU/ml) |
| EC64405 |
Ikhithi Yokuhlola ye-Bioendo EC Endotoxin (I-End-point Chromogenic Assay), 64 Izivivinyo/Ikhithi
| 2 Lyophilized Amebocyte Lysate, 1.7ml/vial; 2 Amanzi e-BET, 50ml/vial; 2 CSE; I-4 Chromogenic Substrate, 1.7ml/vial; | 0.1 – 1 EU/ml |
| I-EC64405S | 0.01 – 0.1 EU/ml; 0.1 – 1 EU/m |
I-Lyophilized Amebocyte Lysate / LAL Reagent ekhiqizwa yi-Bioendo yenziwe ngegazi le-amebocyte lysate elitholakala kunkalankala yehhashi.
| Ikhathalogi No. | Incazelo | Okuqukethwe Kwekhithi | Ukuzwela kwe-EU/ml |
| EC80545 | Ikhithi Yokuhlola I-Bioendo EC Endotoxin (I-End-point Chromogenic Assay, Diazo Coupling), Izivivinyo/Ikhithi engama-80 | 5 I-Lyophilized Amebocyte Lysate, 1.7ml/vial; 4 Amanzi e-BET, 50ml/vial; 5 CSE; I-5 Chromogenic Substrate, 1.7ml/vial; 5 Diazo Reagent 1, 10ml/vial; 5 Diazo Reagent 2, 10ml / vial; 5 Diazo Reagent 3, 10ml/vial; | 0.1 – 1 EU/ml |
| I-EC80545S | 0.01 – 0.1 EU/ml; 0.1 – 1 EU/ml |
Isikhathi sokuthumela: Sep-15-2019
