Ekusebenzeni kwei-bacterial endotoxin test assay, ukusetshenziswa kwamanzi angenayo i-endotoxin kubalulekile ukuze kugwenywe ukungcola.Ukuba khona kwama-endotoxins emanzini kungaholela emiphumeleni engalungile kanye nemiphumela yokuhlola esengozini.Kulapho kungena khona amanzi e-reagent ye-Lyophilized Amebocyte Lysate (LAL) kanye namanzi okuhlolwa kwe-bacterial endotoxin (BET).Lawa manzi aklanywe ngokukhethekile abalulekile ekuqinisekiseni ukwethembeka kanye nokunemba kokuhlolwa kwe-endotoxin ezimbonini ezihlukahlukene, okuhlanganisa ezemithi, izisetshenziswa zezokwelapha, amalabhorethri ocwaningo nokunye.
ILAL amanzi reagentamanzi ahlanzeke kakhulu aklanyelwe ukusetshenziswa ekuhlolweni kwe-LAL kwama-endotoxins.La manzi enza inqubo eqinile yokukhiqiza ukuqinisekisa ukuthi awanawo ama-endotoxins, okungenzeka aphazamise imiphumela yokuhlolwa.Ukungabikho kwama-endotoxins emanzini asebenza nge-LAL kubalulekile ekuqinisekiseni ukuzwela kanye nokucaciswa kokuhlolwa kwe-LAL, okwenza kube ukukhetha okuhle kokutholwa kwe-endotoxin.
Ngokufanayo, amanzi e-BET nawo ayingxenye ebalulekile ekuhlolweni kwe-bacterial endotoxin test.La manzi alungiswa futhi ahlolwe ukuze kuqinisekiswe ukuthi awanawo ama-endotoxin nokunye ukungcola okungase kube nomthelela ekunembeni kokuhlolwa.Ukusebenzisa amanzi e-BET ekuhlolweni kokuhlolwa kwe-endotoxin kubalulekile ukuze uthole imiphumela enokwethenjelwa futhi ekwazi ukukhiqizwa kabusha, njengoba iqeda ingozi yezinto ezingelona iqiniso noma izinto ezingalungile ezingase zenzeke ngenxa yokuba khona kwe-endotoxin emanzini avamile.
Ukubaluleka kokusebenzisa amanzi angenayo i-endotoxin ekuhlolweni kokuhlolwa kwe-endotoxin angeke kweqiswe.Ukunemba nokuthembeka kwemiphumela yokuhlolwa kuncike eqophelweni lamanzi asetshenzisiwe.Ukuba khona kwama-endotoxin emanzini kungaholela ekufundweni okungamanga, okungaba nemiphumela emibi ezimbonini lapho ukuhlolwa kwe-endotoxin kubalulekile ukuze kuqinisekiswe ukuphepha nokusebenza kwemikhiqizo.Ngakho-ke, ukutshala imali emanzini asebenza nge-LAL noma emanzini e-BET kubalulekile ekugcineni ubuqotho benqubo yokuhlola i-endotoxin nokuqinisekisa ikhwalithi nokuphepha kwemikhiqizo.
Sengiphetha, ukusetshenziswa kwamanzi angenayo i-endotoxin, njengamanzi e-LAL reagent namanzi e-BET, kubalulekile ekusebenzeni kokuhlolwa kwe-endotoxin yebhaktheriya.Lawa manzi akhiwe ngokukhethekile aklanyelwe ukuqeda ubungozi bokungcola futhi aqinisekise ukunemba nokuthembeka kokuhlolwa kwe-endotoxin.Ngokusebenzisa la manzi, izimboni zingenza ngokuzethemba ukuhlolwa kwe-endotoxin ngaphandle kokwesaba imiphumela engalungile ngenxa yokuba khona kwe-endotoxin emanzini.Ekugcineni, ukusetshenziswa kwamanzi e-agent ye-LAL namanzi e-BET kubalulekile ukuze kugcinwe izindinganiso eziphakeme zekhwalithi nokuphepha ezimbonini lapho ukuhlolwa kwe-endotoxin kubaluleke kakhulu.
Lapho wenza ukuhlolwa kokuhlolwa kwe-endotoxin yebhaktheriya, kubalulekile ukusebenzisa amanzi angenayo i-endotoxin ukuze uqinisekise imiphumela enembile nethembekile.
Ama-endotoxins ayizingxenye ezizinzile zokushisa odongeni lweseli lama-bacterium angenayo igram, futhi angabangela imfiva, ukushaqeka, ngisho nokufa kubantu nasezilwaneni.
Ngakho-ke, kubalulekile ukusebenzisa amanzi angenayo i-endotoxins lapho wenza ukuhlolwa.
Kunezinhlobo ezimbalwa zamanzi ezingasetshenziswa ekuhlolweni kwe-endotoxin yebhaktheriya, okuhlanganisa amanzi asebenza nge-LAL, amanzi asebenza nge-TAL, namanzi anokwelashwa kwe-depyrogenation.Ngayinye yalezi zinhlobo zamanzi iklanyelwe ukuqinisekisa ukuthi i-endotoxins ayikho, ngaleyo ndlela iqinisekisa ukunemba kwemiphumela yokuhlola.
Amanzi e-LAL reagent amanzi ahlolwe ngokukhethekile futhi aqinisekiswa ukuthi awanawo ama-endotoxins.La manzi avame ukusetshenziswa ekuhlolweni kwe-Lyophilized Amebocyte Lysate (LAL), okuyindlela evame kakhulu yokuthola ubuthi be-endotoxin.Ngokusebenzisa amanzi e-LAL reagent ekuhlolweni, abacwaningi bangaqiniseka ukuthi amanzi ngokwawo awabandakanyi kunoma yimiphi imiphumela engalungile noma engalungile engamanga.
Ngokufanayo, amanzi asebenza nge-TAL angamanzi ahlolwe ngokukhethekile futhi aqinisekiswa ukuthi awanawo ama-endotoxin.La manzi avame ukusetshenziswa ekuhlolweni kwe-Tachypleus Amebocyte Lysate (TAL), enye indlela evamile yokuthola ama-endotoxins.Ngokusebenzisa amanzi e-TAL reagent ekuhlolweni, abacwaningi bangaqiniseka ukuthi amanzi ngokwawo awabandakanyi kunoma yimiphi imiphumela engalungile noma engalungile engamanga.
Amanzi anokwelashwa kwe-depyrogenation angenye inketho yokuqinisekisa ukuthi amanzi asetshenziswa ekuhloleni i-endotoxin yebhaktheriya awanawo ama-endotoxin.Ukwelashwa kwe-Depyrogenation kuhilela ukukhishwa noma ukungasebenzi kwama-pyrogens, kuhlanganise nama-endotoxins, emanzini.Lokhu kungafezwa ngezinqubo ezifana nokuhlunga, ukucwiliswa kwe-distillation, noma ukwelashwa ngamakhemikhali.Ngokusebenzisa amanzi ngokwelashwa kwe-depyrogenation ekuhlolweni, abacwaningi bangaqiniseka ukuthi amanzi ngokwawo awabandakanyi kunoma yimiphi imiphumela engalungile noma engalungile engamanga.
Ngakho-ke, kungani kubaluleke kangaka ukusebenzisa amanzi angenayo i-endotoxin ekuhlolweni kwe-bacterial endotoxin test?Ukuba khona kwama-endotoxins emanzini asetshenziswa ekuhlolweni kungaholela emiphumeleni engalungile, engaba nemiphumela engathi sína kukho kokubili ucwaningo kanye nezicelo zomtholampilo.Isibonelo, uma ama-endotoxins ekhona emanzini, angaholela emiphumeleni emihle engamanga, ebonisa ukuba khona kwama-endotoxins lapho engekho ngempela.Lokhu kungaholela ekukhathazekeni okungadingekile kanye nokusetshenziswa kabi kwezinsiza okungase kube khona ukulungisa inkinga engekho ngempela.
Ngokuphambene, uma ama-endotoxins ekhona emanzini futhi engabonakali, angaholela emiphumeleni engemihle engamanga, ebonisa ukuthi i-endotoxins ayikho lapho ikhona ngempela.Lokhu kungaholela ekukhishweni kwemikhiqizo engcolile, okubeka impilo yabantu neyezilwane engcupheni.
Ngokungeziwe kumthelela ongaba khona ekunembeni kwemiphumela yokuhlolwa, ukusebenzisa amanzi angenayo i-endotoxin nakho kungaphazamisa ukusebenza kohlolo ngokwalo.Ama-endotoxins angaphazamisa ama-reagents nemishini esetshenziswa ekuhlolweni, okuholela emiphumeleni engathembeki noma engahambisani.Ngokusebenzisa amanzi angenayo i-endotoxin, abacwaningi banganciphisa lezi zingozi futhi baqinisekise ukuthi ukuhlolwa kwenziwa ngaphansi kwezimo ezinokwethenjelwa kakhulu.
Ekugcineni, ukuqinisekisa ukuthi amanzi asetshenziswa ekuhlolweni kwe-endotoxin yebhaktheriya awanawo ama-endotoxins kubalulekile ekugcineni ubuqotho bemiphumela yokuhlola.Kungakhathaliseki ukuthi usebenzisa amanzi asebenza nge-LAL, amanzi e-TAL reagent, noma amanzi anokwelashwa kwe-depyrogenation, abacwaningi bangathatha izinyathelo ezisheshayo ukuze baqinisekise ukuthi amanzi awabambi iqhaza kunoma yikuphi ukungalungi noma ukungahambisani emiphumeleni yokuhlola.Ngokwenza kanjalo, bangaba nokuqiniseka ekuqinisekiseni lokho abakutholile futhi bangenza izinqumo ezinolwazi ngokusekelwe emiphumeleni yokuhlolwa.
Isikhathi sokuthumela: Jan-26-2024
