I-Endpoint Chromogenic Kit EC64405
Ikhithi yokuhlola ye-Chromogenic Endotoxin yephoyinti lokugcina (ngaphandle kwe-Diazo Coupling)
1. Ulwazi Lomkhiqizo
Ikhithi Yokuhlola Ye-Chromogenic Endotoxin Yokuphela(ngaphandle kwe-Diazo Coupling) yenziwa ngokwengeza isixazululo se-peptide yokwenziwa engenambala exubeni le-Lyophilized Amebocyte Lysate kanye nesampula yokuhlola ngemva kwesikhathi esithile sokufukamela.Uma isampula yokuhlola iqukethe i-endotoxin, umbala ophuzi uzovela ku-96 we-microplate yomthombo.Ukumunca kwayo (λmax = 405nm) kuhlobene nokuhlushwa kwe-endotoxin.Ukugxiliswa kwe-endotoxin kwesampula yokuhlola kungabalwa ngokusekelwe kwijika elijwayelekile.
2. Ipharamitha yomkhiqizo
Ibanga Lokuzwela: 0.01-0.1 EU/ml, isikhathi sokuhlola okufanayo cishe imizuzu engama-46
0.1-1 EU/ml, isikhathi sokuhlola okufanayo cishe imizuzu eyi-16.
3. Izici Zomkhiqizo kanye Nokusebenza
Ikhithi Yokuhlola I-Chromogenic Endotoxin Yephoyinti Lokugcina (ngaphandle kwe-Diazo Coupling) ihloselwe ukusetshenziswa ekutholweni kwe-In Vitro kanye nenani lama-endotoxin ebhaktheriya e-gram-negative.Isixazululo se-peptide substrate esingenambala esingenambala siyengezwa engxubeni ye-Lyophilized Amebocyte Lysate kanye nesampula yokuhlola ngemva kwesikhathi esithile sokufukamela.Umbala ophuzi uyakhula uma isampula yokuhlola iqukethe i-endotoxin.Bese usebenzisa i-spectrophotometer evamile noma isifundi sepuleti ukuze ufunde ukumuncwa kokuthi 405nm.Ikhithi Yokuhlola I-Chromogenic Endotoxin Yokuphela (ngaphandle kwe-Diazo Coupling) ifanele zonke izinhlobo zamasampula ezinto eziphilayo njengeprotheni, umgomo, i-plasmid, i-DNA, i-RNA endotoxin assay.
Qaphela:
I-reagent ye-Lyophilized Amebocyte Lysate (LAL) ekhiqizwa yi-Bioendo yenziwe ngegazi elitholakala nge-amebocyte lysate lenkalankala yehhashi.
| Ikhathalogi No. | Incazelo | Okuqukethwe Kwekhithi | Ukuzwela (EU/ml) |
| EC64405 |
I-Bioendo™ EC Endotoxin Test Kit (I-End-point Chromogenic Assay), 64 Izivivinyo/Ikhithi
| 2 Lyophilized Amebocyte Lysate, 1.7ml/vial; 2 Amanzi e-BET, 50ml/vial; 2 CSE; I-4 Chromogenic Substrate, 1.7ml/vial; | 0.1 – 1 EU/ml |
| I-EC64405S | 0.01 – 0.1 EU/ml; 0.1 – 1 EU/m |
Ukuzwela kwe-Lyophilized Amebocyte Lysate kanye namandla e-Control Standard Endotoxin kuhlolwa ngokumelene ne-USP Reference Standard Endotoxin.I-Lyophilized Amebocyte Lysate reagent kits iza nemiyalelo yomkhiqizo, Isitifiketi Sokuhlaziya.
Ingabe ikhithi yokuhlola i-Endotoxin yephoyinti lokugcina idinga isifundi se-microplate esiyinkimbinkimbi?
Ukuhlaziywa kwekhithi ye-Bioendo EC64405 kanye ne-EC64405S imiphumela ye-endotoxin yobuningi ngokusebenzisa isifundi se-microplate esivamile.
I-Endotoxin Free Tube
I-Pyrogen Free Tips
I-Pyrogen Free Microplates
Umfundi weMicroplate ojwayelekile
I-endotoxin ejwayelekile yokulawula (CSE10V)
Ukusetshenziswa kwamanzi BET ukuze kwakhiwe kabusha.
Shiya Imilayezo Yakho
Imikhiqizo ehlobene
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I-Endpoint Chromogenic Kit EC80545
Ikhithi Yokuhlola I-Bioendo EC Endotoxin (I-End-point Chromogenic Assay, I-Diazo Coupling) 1. Ulwazi Lomkhiqizo I-Bioendo EC Endotoxin Test Kit (I-End-point Chromogenic Assay, i-Diazo Coupling) inikeza ukulinganisa okusheshayo komthamo we-endotoxin.I-endotoxin esesampuleni yenza kusebenze ingxubevange yama-enzyme ku-Amebocyte Lysate, i-enzyme ecushiwe ihlukanisa i-substrate yokwenziwa, ikhiphe into enombala ophuzi.Khona-ke into ephuzi ingasabela ngokuqhubekayo ngama-diazo reagent ukuze yenze izinto ezinsomi ezine-abso ephezulu...
