I-Endpoint Chromogenic Kit EC80545
Ikhithi Yokuhlola ye-Bioendo EC Endotoxin (I-End-point Chromogenic Assay, I-Diazo Coupling)
1. Ulwazi Lomkhiqizo
Ikhithi Yokuhlola I-Bioendo EC Endotoxin (I-End-point Chromogenic Assay, i-Diazo Coupling) inikeza ukulinganisa okusheshayo komthamo we-endotoxin.I-endotoxin esesampuleni yenza kusebenze ingxubevange yama-enzyme ku-Amebocyte Lysate, i-enzyme ecushiwe ihlukanisa i-substrate yokwenziwa, ikhiphe into enombala ophuzi.Khona-ke into ephuzi ingakwazi ukuqhubeka isabela ngama-reagents e-diazo ukuze yenze izinto ezinsomi ezinokumunca okuphezulu ku-545nm.I-spectrophotometer evamile noma isifundi se-microplate siyadingeka ekuhlolweni.Izinto ezinsomi zilingana nokuhlushwa kwe-endotoxin.Ngemuva kwalokho umphumela wokuhlolwa kwe-endotoxin uwukuhlaziywa komthamo.
2. Ipharamitha yomkhiqizo
Ibanga Lokuzwela: 0.01-0.1EU/ml (isikhathi sokuhlola cishe imizuzu engama-46)
0.1-1.0EU/ml (isikhathi sokuhlolwa cishe imizuzu eyi-16)
3. Izici Zomkhiqizo kanye Nokusebenza
Ikhithi Yokuhlola I-Bioendo EC Endotoxin (I-End-point Chromogenic Assay, i-Diazo Coupling) ihloselwe ukusetshenziswa ekutholweni kwe-In Vitro kanye nenani lama-endotoxin ebhaktheriya e-gram-negative.Isixazululo esingenambala se-peptide substrate sengezwa ku-Lyophilized Amebocyte Lysate, bese sihlola ingxube yesampula.Uma isampula iqukethe i-endotoxin, umbala wengxube yesampula uyashintsha.Ukumuncwa kuhlobene nokuhlushwa kwe-endotoxin.Ngakho-ke amazinga e-endotoxn kungxube yesampula angacaciswa ngokumelene nejika elijwayelekile.I-spectrophotometer evamile enokuhlunga okungu-540 – 545nm yanele ukukala i-endotoxin ngekhithi yethu yokuhlola ye-EC Endotoxin (I-End-point Chromogenic Assay, i-Diazo Coupling).
Qaphela:
I-reagent ye-Lyophilized Amebocyte Lysate (LAL) ekhiqizwa yi-Bioendo yenziwe ngegazi elitholakala nge-amebocyte lysate lenkalankala yehhashi.
| Ikhathalogi No. | Incazelo | Okuqukethwe Kwekhithi | Ukuzwela kwe-EU/ml |
| EC80545 | I-Bioendo™ EC Endotoxin Test Kit (I-End-point Chromogenic Assay, Diazo Coupling), Izivivinyo/Ikhithi engama-80 | 5 I-Lyophilized Amebocyte Lysate, 1.7ml/vial; 4Amanzi e-BET, 50ml/vial; 5 CSE; I-5 Chromogenic Substrate, 1.7ml/vial; 5 Diazo Reagent 1, 10ml/vial; 5 Diazo Reagent 2, 10ml / vial; 5 Diazo Reagent 3, 10ml/vial; | 0.1 – 1 EU/ml |
| I-EC80545S | 0.01 – 0.1 EU/ml; 0.1 – 1 EU/ml |
Ukuzwela kwe-Lyophilized Amebocyte Lysate kanye namandla e-Control Standard Endotoxin kuhlolwa ngokumelene ne-USP Reference Standard Endotoxin.I-Lyophilized Amebocyte Lysate reagent kits iza nemiyalelo yomkhiqizo, Isitifiketi Sokuhlaziya.
Ingabe ikhithi yokuhlola i-Endotoxin yephoyinti lokugcina idinga isifundi se-microplate esiyinkimbinkimbi?
I-Bioendo EC80545 ne-EC80545S, ingafundwa nge-spectrophotometer evamile.
Shiya Imilayezo Yakho
Imikhiqizo ehlobene
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I-Endpoint Chromogenic Kit EC64405
Ikhithi Yokuhlola I-Chromogenic Endotoxin Ephelele (ngaphandle kwe-Diazo Coupling) 1. Ulwazi Lomkhiqizo Indawo Yokugcina Ikhithi Yokuhlola I-Chromogenic Endotoxin (ngaphandle kwe-Diazo Coupling) yenziwa ngokwengeza isixazululo esingenambala sokwenziwa se-peptide substrate ibe yingxube ye-Lyophilized Amebocyte Lysate kanye nesampula yokuhlola ngemva kwesampula ethile. inkathi yokufukanyelwa.Uma isampula yokuhlola iqukethe i-endotoxin, umbala ophuzi uzovela ku-96 we-microplate yomthombo.Ukumunca kwayo (λmax = 405nm) kuhlobene nokuhlushwa kwe-endotoxin.I-Endotoxin ...
